Diagnostic performance of the rapid test for the detection of NS1 antigen and IgM and IgG anti-antibodies against dengue virus

Authors

DOI:

https://doi.org/10.17843/rpmesp.2022.394.11471

Keywords:

Dengue, NS1 Antigen, IgM, IgG, Rapid Test, Diagnosis, Peru, DENV

Abstract

Objectives. To assess the diagnostic performance of the SD dengue DUO rapid test (Inyecta) for the detection of NS1, IgM and IgG in comparison to the ELISA test. Materials and methods. This is a diagnostic test evaluation that included 286 serum samples from patients with symptomatology attributable to dengue from endemic areas of Peru. The samples were analyzed by ELISA and the SD dengue DUO rapid test (Inyecta) for IgM, NS1 and IgG at the Instituto de Investigación Nutricional in Lima. Results. The sensitivity of the rapid test was 68.0% for NS1 and IgM, and 86.0% for IgG, improving to 75.0% and 81.0% for NS1 and IgM, respectively, during the first three days. The specificity for all three analytes was greater than 87.0%. The concordance of the results, measured by the Kappa coefficient for the three analytes, was good and no cross-reaction with other arboviruses was found. Conclusions. The SD dengue DUO rapid test allows detection of NS1, IgM and IgG with adequate sensitivity and specificity. Sensitivity for IgM and NS1 increases when detected during the first three days of symptoms. Therefore, we recommend its implementation in primary care centers for early and timely diagnosis.

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Author Biography

  • Johanna Martins-Luna, Universidad Peruana de Ciencias Aplicadas, Lima, Perú

     

     

References

Organización Mundial de la Salud. Dengue [Internet]. (Consultado el 12 de junio del 2020). Disponible en: https://www.who.int/topics/dengue/es/.

Pan American Health Organization. Dengue cases [Internet]. (Consultado el 12 de junio del 2020). Disponible en: https://www.paho.org/data/index.php/en/mnu-topics/indicadores-dengue-en/dengue-nacional-en/252-dengue-pais-ano-en.html.

Centro Nacional de Epidemiología, Prevención y Control de Enfermedades. Sala situacional Dengue [Internet]. (Consultado el 12 de

junio del 2020). Disponible en: https://www.dge.gob.pe/portal/index.php?option=com_content&view=article&id=14:sala-situacional&catid=8:fortalecimiento-institucional&Itemid=121.

Cabezas C, Fiestas V, García-Mendoza M, Palomino M, Mamani E, Donaires F. Dengue en el Perú: a un cuarto de siglo de su reemergencia. Rev Peru Med Exp Salud Publica. 2015;32(1):146-56.

Tang KF, Ooi EE. Diagnosis of dengue: an update. Expert Rev Anti Infect Ther. 2012;10(8):895-907. doi: 10.1586/eri.12.76.

Ministerio de salud. Norma técnica de salud para la vigilancia epidemiológica y diagnóstico de laboratorio de dengue, chikungunya, zika y otras arbovirosis en el Perú. 2016 diciembre 21.

Palomares-Reyes C, Silva-Caso W, del Valle LJ, Aguilar-Luisa MA, Weilg C, et al. Dengue diagnosis in an endemic area of Peru: Clinical characteristics and positive frequencies by RT-PCR and serology for NS1, IgM, and IgG. Int J Infect Dis. 2019;81:31-37. doi: 10.1016/j.ijid.2019.01.022.

Cameron P. Simmons, Jeremy J. Farrar, Nguyen van Vinh Chau, Bridget Wills. Dengue. New England Journal of Medicine. 2012; 366: 1423-1432

Chung-Hao Huang, Li-Li Kuo, Kuender D. Yang, Po-Shan Lin, Po-Liang Lu, Chien-Chou Lin, et al. Laboratory diagnostics of dengue fever: An emphasis on the role of comercial dengue virus nonstructural protein 1 antigen rapid test. J Microbiol Immunol Infect. 2013;46(5):358-65. doi: 10.1016/j.jmii.2012.07.011.

Valdez S, Ruiz A, Vázquez R, Calzada G, Guzmán T. Evaluación del sistema diagnóstico SD Dengue Duo para la detección de la proteína NS1 y los anticuerpos IgM e IgG anti-dengue. Rev Cubana Med Trop. 2012;64(1):27-34.

Clemen G, Angel J, Montes C, Tovar JR, Osorio L. Contribución de la prueba rápida NS1 e IgM al diagnóstico de dengue en Colombia en el periodo pre-zika. Infect. 2019;23(3):259-265. doi: 10.22354/in.v23i3.790.

SD BIOLINE Dengue Duo Simultaneous Dengue NS1 Ag & IgG/IgM Ab test. Brochure. (Consultado 6 de diciembre de 2022). Disponible en: https://maxanim.com/content/abbott/sd-bioline-dengue-duo.pdf.

Valencia Manosalva DE. Sensibilidad y especificidad para el diagnóstico de dengue de la Prueba rápida SD Bioline Dengue Duo en comparación con el Test de Elisa [tesis de maestría]. Lambayeque: Universidad Nacional Pedro Ruiz Gallo; 2013. Disponible en: https://hdl.handle.net/20.500.12893/7982.

A. Rodriguez. Validación comparada de la prueba rápida SD bioline dengue DUO con el método Gold Stándar (pcr-tiempo real), para el diagnóstico de dengue en fase febril en muestras procedentes de la Región La Libertad 2017. Universidad Nacional de Trujillo 2019,

Blessmann J, Winkelmann Y, Keoviengkhone L, Sopraseuth V, Kann S, Hansenet J, et al. Assessment of diagnostic and analytic performance of the SD Bioline Dengue Duo test for dengue virus (DENV) infections in an endemic area (Savannakhet province, Lao People’s Democratic Republic). PLoS One. 2020;15(3):e0230337. doi: 10.1371/journal.pone.0230337.

Fuente-Alva CS, Molina Villagra M. Likelihood ratio (razón de verosimilitud): definición y aplicación en Radiología. Rev Argent Radiol.

;81(3):204-208. doi: 10.1016/j.rard.2016.11.002.

Sáenz Bolaños E, Lara A, Sequeira S, Alfaro O. Evaluación de una prueba rápida para diagnóstico de dengue en el nivel local. Acta Med Costarric. 2008; 50(4): 20-237.

Parikh R, Parikh S, Arun E, Thomas R. Likelihood ratios: clinical application in day-to-day practice. Indian J Ophthalmol. 2009;57(3):217-21. doi: 10.4103/0301-4738.49397.

Aguilar-Luis MA, Del Valle-Mendoza J, Silva-Caso W, Gil-Ramirez T, Levy-Blitchtein S, Bazán-Mayra J, et al. An emerging public health threat: Mayaro virus increases its distribution in Peru. Int J Infect Dis. 2020 Mar; 92:253-258. doi: 10.1016/j.ijid.2020.01.024.

Published

2022-12-23

Issue

Section

Original Article

How to Cite

1.
Valdivia-Conroy B, Vasquez-Calderón JM, Silva-Caso W, Martins-Luna J, Aguilar-Luis MA, del Valle-Mendoza J, et al. Diagnostic performance of the rapid test for the detection of NS1 antigen and IgM and IgG anti-antibodies against dengue virus. Rev Peru Med Exp Salud Publica [Internet]. 2022 Dec. 23 [cited 2024 Dec. 13];39(4):434-41. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/11471