Detección molecular de infección natural por parásitos tripanosomátidos en Didelphis marsupialis de una zona rural del norte de Colombia

Marlon M. Ardila; Yoselin Villadiego; Leidi Herrera; Wendy Zabala-Monterroza; Alveiro Pérez-Doria

doi:10.17843/rpmesp.2023.401.11573

Authors

Marlon M. Ardila Facultad de Ciencias Básicas y Biomédicas, Universidad Simón Bolívar, Barranquilla, Colombia. Departamento de Ciencia Animal, Facultad de Ciencias Veterinarias, Universidad de Concepción, Chillán, Chile. https://orcid.org/0000-0002-1851-5307
Yoselin Villadiego Grupo Interdisciplinario en Ciencias Marinas y Ambientales (GICMARA), Facultad de Ciencias Básicas, Universidad del Atlántico, Puerto Colombia, Colombia. https://orcid.org/0000-0001-7728-4898
Herrera Herrera Centro de Ecología y Evolución, Instituto de Zoología y Ecología Tropical (IZET), Facultad de Ciencias, Universidad Central de Venezuela (UCV), Caracas, Venezuela. Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, San Lorenzo, Paraguay. https://orcid.org/0000-0001-8515-2078
Wendy Zabala-Monterroza Grupo Investigaciones Biomédicas, Facultad de Educación y Ciencias, Universidad de Sucre, Sincelejo, Colombia. https://orcid.org/0000-0003-3841-5204
Alveiro Pérez-Doria Grupo Investigaciones Biomédicas, Facultad de Educación y Ciencias, Universidad de Sucre, Sincelejo, Colombia. División de Investigación, innovación y desarrollo, Pyrogen S.A.S, Sucre, Colombia. https://orcid.org/0000-0003-1473-5895

DOI:

https://doi.org/10.17843/rpmesp.2023.401.11573

Keywords:

Leishmaniasis, Parasites, Didelphis, Colombia, DNA, Kinetoplast

Abstract

We studied the prevalence of infection by trypanosomatid parasites in Didelphis marsupialis and its relationship with morphological/age aspects in a rural area of El Carmen de Bolivar, Colombia. Five visits were made to the Vereda El Alférez; each of which lasted three consecutive nights. During these visits, Tomahawk® traps were installed in the peridomestic and wild ecotopes of the Vereda El Alférez. Body measurements, sex and age were determined from the collected animals. Blood was extracted by cardiopuncture, after sedation, in order to obtain total deoxyribonucleic acid (DNA) and amplify the conserved region of the kinetoplast minicircle DNA (kDNA) of parasitic trypanosomatids. The association between morphological parameters of didelphids and their frequency of infection by parasitic trypanosomatids was determined by binomial regression. Thirty D. marsupialis specimens (60.0% females and 40.0% males/66.7% adults and 33.3% juveniles) were collected. Molecular diagnosis revealed a frequency of trypanosomatid parasite infection of 46.7%. Stage (p=0.024) was a determinant for infection. We discuss the role of D. marsupialis as a potential reservoir of parasitic trypanosomatids in the Vereda El Alférez.

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