Carbapenems and colistin resistance genes isolated in Musca domestica from a garbage dump near a hospital in Lima

Authors

  • Miguel A. Alarcón-Calle Laboratorio de Investigación en Biología, Facultad de Ciencias Naturales y Matemática, Universidad Nacional Federico Villarreal, Lima, Perú https://orcid.org/0000-0002-9383-7832
  • Víctor L. Osorio-Guevara Laboratorio de Investigación en Biología, Facultad de Ciencias Naturales y Matemática, Universidad Nacional Federico Villarreal, Lima, Perú https://orcid.org/0000-0001-5075-8053
  • Ramsés Salas-Asencios Laboratorio de Biotecnología, Facultad de Ciencias Naturales y Matemática, Universidad Nacional Federico Villarreal, Lima, Perú https://orcid.org/0000-0002-4075-1736
  • José Yareta Laboratorio de Investigación en Biología Molecular, Escuela Profesional de Medicina, Universidad Peruana Unión, Lima, Perú https://orcid.org/0000-0002-8909-1724
  • Pool Marcos-Carbajal Laboratorio de Epidemiología Molecular y Genómica, Instituto de Investigación en Ciencias Biomédicas, Facultad de Medicina Humana, Universidad Ricardo Palma, Lima, Perú https://orcid.org/0000-0002-7741-0337
  • María E. Rodrigo-Rojas Laboratorio de Investigación en Biología, Facultad de Ciencias Naturales y Matemática, Universidad Nacional Federico Villarreal, Lima, Perú https://orcid.org/0000-0002-1555-4036

DOI:

https://doi.org/10.17843/rpmesp.2024.412.13257

Keywords:

Houseflies, Drug Resistance, Colistin, Carbapenem

Abstract

The objective was to determine the presence of genes resistant to carbapenems and plasmid resistance to colistin (mcr-1) in bacteria isolated from Musca domestica in a garbage dump near a hospital in Lima-Peru. Isolation of carbapenemase-producing bacteria was performed in CHROMagar mSuperCARBATM medium and colistin resistance profiling was performed by colistin discs elution method. Detection of blaKPC, blaNDM, blaIMP, blaOXA-48, blaVIM y mcr-1 genes was performed by conventional PCR. Antimicrobial susceptibility profile assessment was performed on the MicroScan automated system. Carbapenemase-producing bacteria were 31/38 and bacteria that were  resistant to colistin were 26/38 with an MIC ≥ 4 μg/mL. Finally, 7 bacterial strains with carbapenem resistance genes (OXA-48 and KPC) and one bacterial strain with plasmid resistance to colistin (mcr-1) were identified.

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References

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Published

2024-06-21

Issue

Section

Brief Report

How to Cite

1.
Alarcón-Calle MA, Osorio-Guevara VL, Salas-Asencios R, Yareta J, Marcos-Carbajal P, Rodrigo-Rojas ME. Carbapenems and colistin resistance genes isolated in Musca domestica from a garbage dump near a hospital in Lima. Rev Peru Med Exp Salud Publica [Internet]. 2024 Jun. 21 [cited 2024 Dec. 12];41(2):164-70. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/13257

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