Genes resistentes a carbapenémicos y colistina aislados en Musca domestica proveniente de un basural cercano a un hospital de Lima

Miguel A. Alarcón-Calle; Víctor L. Osorio-Guevara; Ramsés Salas-Asencios; José Yareta; Pool Marcos-Carbajal; María E. Rodrigo-Rojas

doi:10.17843/rpmesp.2024.412.13257

Authors

Miguel A. Alarcón-Calle Laboratorio de Investigación en Biología, Facultad de Ciencias Naturales y Matemática, Universidad Nacional Federico Villarreal, Lima, Perú https://orcid.org/0000-0002-9383-7832
Víctor L. Osorio-Guevara Laboratorio de Investigación en Biología, Facultad de Ciencias Naturales y Matemática, Universidad Nacional Federico Villarreal, Lima, Perú https://orcid.org/0000-0001-5075-8053
Ramsés Salas-Asencios Laboratorio de Biotecnología, Facultad de Ciencias Naturales y Matemática, Universidad Nacional Federico Villarreal, Lima, Perú https://orcid.org/0000-0002-4075-1736
José Yareta Laboratorio de Investigación en Biología Molecular, Escuela Profesional de Medicina, Universidad Peruana Unión, Lima, Perú https://orcid.org/0000-0002-8909-1724
Pool Marcos-Carbajal Laboratorio de Epidemiología Molecular y Genómica, Instituto de Investigación en Ciencias Biomédicas, Facultad de Medicina Humana, Universidad Ricardo Palma, Lima, Perú https://orcid.org/0000-0002-7741-0337
María E. Rodrigo-Rojas Laboratorio de Investigación en Biología, Facultad de Ciencias Naturales y Matemática, Universidad Nacional Federico Villarreal, Lima, Perú https://orcid.org/0000-0002-1555-4036

DOI:

https://doi.org/10.17843/rpmesp.2024.412.13257

Keywords:

Houseflies, Drug Resistance, Colistin, Carbapenem

Abstract

The objective was to determine the presence of genes resistant to carbapenems and plasmid resistance to colistin (mcr-1) in bacteria isolated from Musca domestica in a garbage dump near a hospital in Lima-Peru. Isolation of carbapenemase-producing bacteria was performed in CHROMagar mSuperCARBATM medium and colistin resistance profiling was performed by colistin discs elution method. Detection of blaKPC, blaNDM, blaIMP, blaOXA-48, blaVIM y mcr-1 genes was performed by conventional PCR. Antimicrobial susceptibility profile assessment was performed on the MicroScan automated system. Carbapenemase-producing bacteria were 31/38 and bacteria that were resistant to colistin were 26/38 with an MIC ≥ 4 μg/mL. Finally, 7 bacterial strains with carbapenem resistance genes (OXA-48 and KPC) and one bacterial strain with plasmid resistance to colistin (mcr-1) were identified.

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