Metformin treatment prevents experimental metabolic syndrome-induced  femoral bone marrow adiposity in rats

Authors

  • Siro Lasalvia Laboratorio de Investigaciones en Osteopatías y Metabolismo Mineral (LIOMM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata. Buenos Aires, Argentina https://orcid.org/0009-0004-7051-1065
  • Claudia Sedlinsky Laboratorio de Investigaciones en Osteopatías y Metabolismo Mineral (LIOMM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata. Buenos Aires, Argentina https://orcid.org/0009-0006-1825-9397
  • León Schurman Laboratorio de Investigaciones en Osteopatías y Metabolismo Mineral (LIOMM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata. Buenos Aires, Argentina https://orcid.org/0009-0008-9614-3036
  • Antonio Desmond McCarthy Laboratorio de Investigaciones en Osteopatías y Metabolismo Mineral (LIOMM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata. Buenos Aires, Argentina https://orcid.org/0000-0002-9175-6596
  • Nahuel Ezequiel Wanionok Laboratorio de Investigaciones en Osteopatías y Metabolismo Mineral (LIOMM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata. Buenos Aires, Argentina https://orcid.org/0009-0006-1614-6292

DOI:

https://doi.org/10.17843/rpmesp.2024.411.13333

Keywords:

Metformin, metabolic syndrome, adipocytes, mesenchymal stem cells, bone tissue

Abstract

Objetive. To determine the effect of metformin (MET) treatment on adipogenic predisposition of bone marrow progenitor cells (BMPC), bone marrow adiposity and  bone biomechanical properties. Materials and methods. 20 young adult male Wistar rats were sorted into four groups. Each of the groups received the following in drinking water: 100% water (C); 20% fructose (F); metformin 100 mg/kg wt/day (M); or fructose plus metformin (FM). After five weeks the animals were sacrificed. Both humeri were dissected to obtain BMPC, and both femurs were dissected to evaluate medullary adiposity (histomorphometry) and biomechanical properties (3-point bending). BMPC were cultured in vitro in adipogenic medium to evaluate RUNX2, PPAR-γ and RAGE expression by RT-PCR, lipase activity and triglyceride accumulation. Results. The fructose-rich diet (group F) caused an increase in both triglycerides in vitro, and medullary adiposity in vivo; being partially or totally prevented by co-treatment with metformin (group FM). No differences were found in femoral biomechanical tests in vivo, nor in lipase activity and RUNX2/PPAR-γ ratio in vitro. DRF increased RAGE expression in BMPC, being prevented by co-treatment with MET. Conclusions. Metabolic syndrome induced by a fructose-rich diet increases femoral medullary adiposity and, in part, the adipogenic predisposition of BMPC. In turn, this can be totally or partially prevented by oral co-treatment with MET.

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References

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Published

2024-05-08

Issue

Section

Original Article

How to Cite

1.
Lasalvia S, Sedlinsky C, Schurman L, McCarthy AD, Wanionok NE. Metformin treatment prevents experimental metabolic syndrome-induced  femoral bone marrow adiposity in rats. Rev Peru Med Exp Salud Publica [Internet]. 2024 May 8 [cited 2024 Nov. 7];41(1):28-36. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/13333