Native antigen of aspergillus fumigatus with useful for immunodiagnosis of aspergilloma

Authors

  • José Casquero Laboratorio de Micología, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo.
  • Flor Urcia Laboratorio de Micología, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo.
  • Elizabeth Sánchez Laboratorio de Zoonosis Parasitaria, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo.

DOI:

https://doi.org/10.17843/rpmesp.2009.262.1356

Keywords:

Aspergillus fumigatus, Immunologic tests, Immunodiffusion, Antigens

Abstract

In order to evaluate the usefulness of native antigens of autochthonous strains of Aspergillus fumigatus for immunodiagnosis of aspergilloma, We was conducted a study using two strains of this fungus, isolated from patients with aspergilloma (533 and 554), which were confronted with commercial control serum of A. fumigatus, A. flavus, A. niger, Candida, Coccidioides, Histoplasma and Paracoccidioides by immunodiffusion test, moreover, we evaluated 28 serum samples from patients with suspected aspergilloma. We also performed the characterization of the protein components of native antigens using the technique of SDS-PAGE and were confronted with ten serum samples from patients with aspergilloma, paracoccidioidomycosis, histoplasmosis, C-reactive protein and hydatidosis, also healthy person serum by immunoblot. Good agreement (kappa 0.92) was found between antigens 533 and 554 and somewhat less than those with commercial antigen A. fumigatus (kappa 0.73 and 0.81 for the 533 and 554, respectively). The band of 97 kDa reacted only with sera from patients with aspergilloma being immunodominant.

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Published

2009-06-30

Issue

Section

Research Articles

How to Cite

1.
Casquero J, Urcia F, Sánchez E. Native antigen of aspergillus fumigatus with useful for immunodiagnosis of aspergilloma. Rev Peru Med Exp Salud Publica [Internet]. 2009 Jun. 30 [cited 2024 Jul. 25];26(2). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/1356

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