Characteristic biological of a strain of Trypanosoma cruzi in a murine model and analysis of survival

Authors

  • Nicolas Suárez Centro de Salud de Colcabamba, Dirección Regional de Salud de Huancavelica, Ministerio de Salud. Huancavelica, Perú. Departamento de Ciencias Biológicas, facultad de Ciencias, universidad Nacional San Luis Gonzaga. Ica, Perú. Biólogo.
  • Rufino Cabrera Grupo Temático de Enfermedades Metaxénicas, Dirección General de Epidemiología, Ministerio de Salud. Lima, Perú. Biólogo.
  • Luis Cartagena Departamento de Ciencias Biológicas, facultad de Ciencias, universidad Nacional San Luis Gonzaga. Ica, Perú. Biólogo.
  • Rolando Ayaqui Departamento de Microbiología y Patología de la facultad de Medicina, universidad Nacional de San Agustín de Arequipa, Perú. Biólogo Doctor en Ciencias Biomédicas.

DOI:

https://doi.org/10.17843/rpmesp.2009.262.1357

Keywords:

Trypanosoma cruzi, Pathogenicity, Mice, Animal disease model, Peru

Abstract

To know the biological characteristics of a strain of Trypanosome cruzi, coming from the district of Tiabaya, Arequipa, Peru, and to determine the survival of inoculated mice relating the infecting dose ante and sex. Were used 14 BALB/C-53 mice (males and females) of 30 days of age as biological model. The groups I and II were inoculated via intraperitoneum with 1,8 x 105 and 1,8 x 104 meta-cyclic trypomastigotes of T. cruzi, respectively; group III with 1,5 x 107 sanguine trypomastigotes. Control group (IV) received only physiologic saline solution at 0.9%. The parasites were searched in peripheral blood of the mouse. The analysis of survival was carried out by Kaplan-Meier method, Logrank test and Wilcoxon to 95 % of confidence level. Were find thin and thick forms of T. cruzi. The fatality rate was 100% groups at day 40 -post-infection- (pi). The parasitemy was detected from the 6th until the 40th day pi and the global maximum pick (6747 parasites) was observed on the 29th day pi. Significant differences exist in the survival of inoculated mice according to the dose by the Logrank test (p = 0,0202). The strain of T. cruzi, coming from Tiabaya, Arequipa is of medium virulence. The survival of the infected mice is related to the dose of inoculation.

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Published

2009-06-30

Issue

Section

Research Articles

How to Cite

1.
Suárez N, Cabrera R, Cartagena L, Ayaqui R. Characteristic biological of a strain of Trypanosoma cruzi in a murine model and analysis of survival. Rev Peru Med Exp Salud Publica [Internet]. 2009 Jun. 30 [cited 2024 Nov. 2];26(2). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/1357

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