Development of the antigenic recombinant tropomyosin of Echinococcus granulosus in a bacterial system as a vaccinal candidate against canine echinococcosis

Janet Acosta-Benites; Luis M.  Jara; Manuela Verastegui Pimentel; Pepe M. Obregón Maldonado; Faride  Altamirano-Zevallos; Nicasio Valencia Mamani; Cesar M. Gavidia

doi:10.17843/rpmesp.2024.414.13854

Authors

Janet Acosta-Benites Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú. https://orcid.org/0009-0001-8509-5752
Luis M. Jara Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima, Perú. https://orcid.org/0000-0002-8770-5657
Manuela Verastegui Pimentel Laboratorio de Investigación de Enfermedades Infecciosas (LIEI), Facultad de Ciencia y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Perú. https://orcid.org/0000-0002-7500-1353
Pepe M. Obregón Maldonado Laboratorio de Investigación de Enfermedades Infecciosas (LIEI), Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Perú https://orcid.org/0000-0002-2322-6091
Faride Altamirano-Zevallos Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Perú https://orcid.org/0000-0003-4348-8571
Nicasio Valencia Mamani Facultad de Ciencias e Ingeniería, Universidad Nacional de Huancavelica, Huancavelica, Perú https://orcid.org/0000-0002-9408-7770
Cesar M. Gavidia Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Perú https://orcid.org/0000-0003-3936-5077

DOI:

https://doi.org/10.17843/rpmesp.2024.414.13854

Keywords:

Recombinant DNA, echinococcosis, Echinococcus granulosus, Tropomyosin, vaccine

Abstract

This study aimed to clone, express and produce the recombinant Echinococcus granulosus tropomyosin
isoform A protein (EgTrpA) that maintains its antigenic and immunogenic properties as a potential vaccine
candidate for dogs and sheep. The Echinococcus granulosus tropomyosin protein (EgTrp) gene was cloned into two vectors: Tropo/His-tag [pET28a (+)] and Tropo/GST-tag (pGEX6P-1). It was then expressed in E. coli BL21. Protein identity was determined by two-dimensional electrophoresis. Immunogenicity and antigenicity were verified by immunizing rabbits with each recombinant protein and assessed by western blot and ELISA. Two-dimensional electrophoresis identified the recombinant EgTrp protein as isoform A. The recombinant proteins showed recognition reactions on Western Blot and serum from immunized rabbits showed an increase in Tropo/His-tag IgG antibodies similar to Tropo/GST-tag. The recombinant EgTrpA protein showed antigenic and immunogenic characteristics in laboratory animals.

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