Caracterización y optimización del antígeno del líquido hidatídico de ovino y su aplicación en la prueba de látex

Flor Fuentes; Nelly Incio; Juan Lévano; Yovanna Torres

doi:10.17843/rpmesp.2009.264.1410

Authors

Flor Fuentes Centro Nacional de Productos Biológicos, Instituto Nacional de Salud. Lima, Perú. Químico farmacéutico.
Nelly Incio Centro Nacional de Productos Biológicos, Instituto Nacional de Salud. Lima, Perú. Químico farmacéutico.
Juan Lévano Centro Nacional de Productos Biológicos, Instituto Nacional de Salud. Lima, Perú. Médico veterinario.
Yovanna Torres SAIS Tupac Amaru, EsSalud. Junín Perú. Médico

DOI:

https://doi.org/10.17843/rpmesp.2009.264.1410

Keywords:

Echinococcosis, diagnosis, Latex Fixation Tests, Agglutination tests

Abstract

It was characterized and optimized sheep hydatid fluid antigen and applied in latex fixation tests as screening test for serological diagnosis of patients with Echinococcus granulosus cysts. We evaluated 40 sera, 15 sera positive by immunoblot from patients with E. granulosus infection, 10 sera from patients with other parasitic diseases and 15 sera from healthy subject. Three of the 15 hydatidosis sera were negative and 0 / 25 sera with hydatidosis were reactive. The sensitivity was 80% (95%CI: 56.4 to 100%), specificity was 100% (96.7 to 100%), positive predictive value of 100% (95.8 to 100%), negative predictive value of 83.3 (30.4 to 69.6%), and repeatability and reproducibility of 100%. We recommended the use of this test for the diagnosis of hydatid disease, because it is simple, fast and reproducible, as a kit in the laboratory or in epidemiological field studies.