Oral toxicity at 60-days of sacha inchi oil (Plukenetia volubilis L.) and linseed (Linum usitatissimum L.), and determination of lethal dose 50 in rodents )

Authors

  • Arilmi Gorriti Laboratorio de farmacognosia y Medicina Tradicional, facultad de farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos. Lima, Perú. Centro Latinoamericano de Enseñanza e Investigación Bacteriológica, facultad de farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos. Lima, Perú. Químico farmacéutico. Doctor en farmacia y Bioquímica.
  • Jorge Arroyo Instituto de Investigaciones Clínicas, facultad de Medicina, Universidad Nacional Mayor de San Marcos. Lima, Perú. Químico farmacéutico.
  • Fredy Quispe Unidad de I-D+I, Empresa Agronegocios Peruagro S.R.L. Arequipa, Perú. Químico farmacéutico. Candidato a Magíster en Química.
  • Braulio Cisneros Instituto de Investigaciones Clínicas, facultad de Medicina, Universidad Nacional Mayor de San Marcos. Lima, Perú. Químico farmacéutico.
  • Martín Condorhuamán Dirección de Salud (DISA) V, Ministerio de Salud. Lima, Perú. Médico Cirujano.
  • Yuan Almora Departamento de farmacia, Hospital Nacional Hipólito Unánue. Lima, Perú. Químico farmacéutico.
  • Víctor Chumpitaz Laboratorio de farmacología, facultad de Odontología, Universidad Nacional Mayor de San Marcos. Lima, Perú. Químico farmacéutico.

DOI:

https://doi.org/10.17843/rpmesp.2010.273.1492

Keywords:

Pharmacology, Plants, medicinal, Toxicity tests, Lethal dose 50

Abstract

Objectives . To evaluate the oral toxicity at 60 days and to determine the lethal dose 50 (LD 50) of raw sacha inchi ( Plukenetia volubilis L .) and linseed ( Linum ussitatisimum ) oils in Holtzman rats and mice of the strain Balb C57 respectively. Materials and methods . f or the evaluation of the oral toxicity of repeated doses for 60 days, 24 male Holtzman rats were used, divided in three groups of 8 each, the groups were: physiologic saline solution 4 mL/kg ( f SS), sacha inchi oil 0.5 mL/kg (SI05) and linseed oil 0.5 mL/kg (L05), during the experiment the body weight was controlled weekly, and signs of toxicity in the research groups, as well as total cholesterol, HDL, glucose, triglycerides and alkaline phosphatase at days 30 and 60 after initiating the experiment. f or the evaluation of the LD50 male mice of the Balb C57 strain were used in groups of 10 animals, and they were administered increasing oral doses of raw oils until reaching 1 mL/kg (37 g/kg). Results . The serum parameters in the rats indicated there is no toxicity at 60 days and that the administration of the oils lowered the levels of cholesterol, triglycerides and increased the HDL in comparison with the control group. The LD50 shows that the raw sacha inchi and linseed oils have doses above 37 g/kg of body weight. Conclusions. Sacha inchi and linseed oils are harmless at 60 days and present a LD50 above the 37 g/kg of animal.

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Published

2010-09-28

Issue

Section

Research Articles

How to Cite

1.
Gorriti A, Arroyo J, Quispe F, Cisneros B, Condorhuamán M, Almora Y, et al. Oral toxicity at 60-days of sacha inchi oil (Plukenetia volubilis L.) and linseed (Linum usitatissimum L.), and determination of lethal dose 50 in rodents ). Rev Peru Med Exp Salud Publica [Internet]. 2010 Sep. 28 [cited 2024 Apr. 21];27(3). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/1492