In vitro antibacterial activity of extracts and purified fractions from the marine sponge Suberites aff. latus (Demospongiae, Suberitida, Suberitidae) from Ica, Peru

Erin Villanueva-Coronado; Juan C.  Francia-Quiroz; Christian Polo; Oscar Reategui; Báslavi  Cóndor-Luján; Aldo G.  Indacochea; Maria J. Pons

doi:10.17843/rpmesp.2025.424.15003

Authors

Erin Villanueva-Coronado Carrera de Biología Marina, Facultad de Ciencias Veterinarias y Biológicas, Universidad Científica del Sur, Lima, Perú https://orcid.org/0000-0003-4852-9897
Juan C. Francia-Quiroz Carrera de Biología Marina, Facultad de Ciencias Veterinarias y Biológicas, Universidad Científica del Sur, Lima, Perú https://orcid.org/0000-0001-5095-6623
Christian Polo Carrera de Biología Marina, Facultad de Ciencias Veterinarias y Biológicas, Universidad Científica del Sur, Lima, Perú https://orcid.org/0000-0001-6775-8156
Oscar Reategui Grupo de Investigación, Caracterización, Transformación y Sustentabilidad de los Recursos Naturales del Perú (CTS Group), Universidad Científica del Sur, Lima, Perú https://orcid.org/0000-0001-9773-2176
Báslavi Cóndor-Luján Departamento de Zoología, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú https://orcid.org/0000-0001-7832-7319
Aldo G. Indacochea Departamento de Zoología, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú https://orcid.org/0000-0001-8502-8755
Maria J. Pons Grupo de Enfermedades Infecciosas (re) emergentes, Universidad Científica del Sur, Lima, Perú https://orcid.org/0000-0001-8384-2315

DOI:

https://doi.org/10.17843/rpmesp.2025.424.15003

Keywords:

Minimum Inhibitory Concentration, Microdilution, Chemical Screening, Paracas, Porifera, Bioactivity

Abstract

Marine sponges constitute a recognized source of metabolites with potential antibacterial activity. In the present study, the antibacterial activity of the marine sponge Suberites aff. latus from Ica, Peru, was evaluated. Lyophilized sponge samples were extracted using dichloromethane:methanol (1:1), and fractionated by solid-phase extraction into seven fractions (F1–F7), ranging from aqueous to organic polarity. The minimum inhibitory concentration was determined against Gram-positive/negative bacterial strains from the ATCC (American Type Culture Collection) collection. The results showed that the highest activity was observed in fraction F6 of specimen 01J against Klebsiella pneumoniae and Pseudomonas aeruginosa, as well as in fraction F3 of specimen 02J against K. pneumoniae. Qualitative chemical analysis revealed the presence of alkaloids and saponins in the samples. These findings highlight the potential of Suberites aff. latus from the Peruvian coast as a promising source of antibacterial compounds.

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