Vasodilatador effect mediated by nitric oxide of the Zea mays L (Andean purple corn) hydroalcoholic extract in aortic rings of rat

Authors

  • Oscar Moreno-Loaiza Facultad de Medicina, Universidad Nacional San Agustín, Arequipa, Perú. Sociedad Científica de Estudiantes de Medicina Agustinos, Arequipa, Perú. Estudiante de medicina.
  • Azael Paz-Aliaga Facultad de Medicina, Universidad Nacional San Agustín, Arequipa, Perú. Centro de Investigación y Desarrollo Científico, Universidad Nacional San Agustín, Arequipa, Perú. Biólogo, PhD en Fisiología y Biofísica.

DOI:

https://doi.org/10.17843/rpmesp.2010.274.1523

Keywords:

Medicinal plants, Antihypertensive agents, Nitric oxide, In vitro

Abstract

Objective: To evaluate the vasodilator response of the hydroalcoholic extract of Zea mays L. (Andean purple corn) and to determine if this response is mediated by nitric oxide (NO). Material and methods: We obtained an extract by maceration for eight days of Andean purple corn cobs in 70% ethanol and subsequent concentration of the product. Thoracic aortic rings were evaluated in an isolated organ chamber, bathed with Krebs-Hensleit solution (KH), and vasomotor activity was recorded with an isometric tension transducer. Basal contraction was produced with 120 mM KCl and then, we proceeded to determinate the vasodilator effect of 3 doses of the extract: 0.1, 0.5, and 1.0 mg/mL. We used L-NG-Nitroarginin methyl ester (L-NAME) to verify that the vasodilation depends on nitric oxide sinteasa (NOs). Then we compared the inhibition of vascular contraction after incubation for 30 minutes, with purple corn extract and captopril 10-5 M. Results: We observed a reduction in maximum contraction (100%) to 85.25 ± 2.60%, 77.76 ± 3.23%, and 73.3 ± 4.87% for doses of 0.1, 0.5 and 1,0 mg/mL respectively. The vasodilation was inhibited by prior incubation with L-NAME. Andean purple corn extract did not inhibit vascular contraction as captopril did (reduction to 75.27 ± 8.61%). Conclusion. The hydroalcoholic extract of Zea mays L produces NO dependent vasodilation.

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Published

2010-12-23

Issue

Section

Research Articles

How to Cite

1.
Moreno-Loaiza O, Paz-Aliaga A. Vasodilatador effect mediated by nitric oxide of the Zea mays L (Andean purple corn) hydroalcoholic extract in aortic rings of rat. Rev Peru Med Exp Salud Publica [Internet]. 2010 Dec. 23 [cited 2024 Jul. 16];27(4). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/1523