Metalo-ß-lactamases in clinical isolates of Pseudomonas aeruginosa in Lima, Peru

Authors

  • Edgar Gonzales-Escalante Instituto Nacional de Salud del Niño. Lima, Perú. Tecnólogo médico.
  • William Vicente-Taboada Instituto Nacional de Enfermedades Neoplásicas. Lima, Perú. médico patólogo clínico.
  • Roky Champi-Merino Hospital Nacional Hipólito Unanue. Lima, Perú. Tecnólogo médico.
  • Javier Soto-Pastrana Hospital Nacional Docente Madre Niño San Bartolomé. Lima, Perú. Tecnólogo médico.
  • Wilfredo Flores-Paredes Hospital Nacional Guillermo Almenara Irigoyen. Lima, Perú. médico patólogo clínico.
  • Margarita Lovera-García Hospital Nacional Guillermo Almenara Irigoyen. Lima, Perú. 6 Hospital Alberto Sabogal Sologuren. Lima, Perú Tecnólogo médico.
  • Nancy Chuquiray-Valverde Hospital Alberto Sabogal Sologuren. Lima, Perú. Tecnólogo médico.
  • Carlos Bejarano-Cristobal Hospital Alberto Sabogal Sologuren. Lima, Perú. Tecnólogo médico.
  • Maritza Puray-Chávez Laboratorio de Epidemiología Molecular y Genética, Instituto de Medicina Tropical, Universidad Nacional Mayor de San Marcos. Lima, Perú. Tecnólogo médico.
  • Segundo León-Sandoval Laboratorio de Epidemiología Molecular y Genética, Instituto de Medicina Tropical, Universidad Nacional Mayor de San Marcos. Lima, Perú. Tecnólogo médico. magister en Enfermedades infecciosas y Tropicales.

DOI:

https://doi.org/10.17843/rpmesp.2013.302.198

Keywords:

Drug resistance, bacterial, Penicillinase, Genes, MDR, ltidrug resistance-associated proteins, Pseudomonas aeruginosa

Abstract

The aim of this study was to detect and characterize molecularly metallo-β-lactamase (MβL) in clinical isolates of Pseudomonas aeruginosa. We carry out a cross sectional study in six publics hospital in Lima on August 2011. 51 isolates of P. aeruginosa resistant to ceftazidime and reduced susceptibility to carbapenemes were evaluated. The phenotypic assay was performed using the approximation method with substrate disks (ceftazidime, imipenem and meropenem) and ethylenediaminetetraacetic acid (EDTA). MβL gene detection was performed using the technique of polymerase chain reaction (PCR) multiplex. Through MβL detected phenotypic method in 15.7% of isolates. Detection of genes revealed the presence of the gene in the 8 isolates blaIMP. The first report of MβL in P. aeruginosa in Peru was described, this should alert the monitoring equipment in the institutions to promote control their spread.

Downloads

Download data is not yet available.

Downloads

Published

2014-02-10

Issue

Section

Research Articles

How to Cite

1.
Gonzales-Escalante E, Vicente-Taboada W, Champi-Merino R, Soto-Pastrana J, Flores-Paredes W, Lovera-García M, et al. Metalo-ß-lactamases in clinical isolates of Pseudomonas aeruginosa in Lima, Peru. Rev Peru Med Exp Salud Publica [Internet]. 2014 Feb. 10 [cited 2024 May 28];30(2). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/198

Most read articles by the same author(s)

1 2 > >>