Genetic variability of Aedes aegypti determined by mitochondrial gene ND4 analysis in eleven endemic areas for dengue in Peru

Authors

  • Pamela Yáñez Universidad Nacional de San Antonio Abad del Cusco. Cusco, Perú. Biólogo.
  • Enrique Mamani Laboratorio de Metaxénicas Virales, Instituto Nacional de Salud. Lima, Perú. Biólogo
  • Jorge Valle Laboratorio de Entomología, Instituto Nacional de Salud. Lima, Perú. Biólogo.
  • María Paquita García Laboratorio de Metaxénicas Virales, Instituto Nacional de Salud. Lima, Perú. tecnólogo médico.
  • Walter León Laboratorio de Entomología, Instituto Nacional de Salud. Lima, Perú. Biólogo.
  • Pablo Villaseca Laboratorio de Entomología, Instituto Nacional de Salud. Lima, Perú. Biólogo.
  • Dina Torres Laboratorio de Metaxénicas Virales, Instituto Nacional de Salud. Lima, Perú. Biólogo.
  • César Cabezas Instituto Nacional de salud. Lima. Perú. médico cirujano.

DOI:

https://doi.org/10.17843/rpmesp.2013.302.199

Keywords:

Aedes, Haplotypes, Pedigree, DNA, Mitochondrial, Peru

Abstract

In order to establish the genetic variability of Aedes aegypti determined by the analysis of the MT-ND4 gene, in eleven endemic regions for dengue in Peru, 51 samples of Ae. Aegypti were tested. The genetic variability was determined through the amplification and sequencing of a fragment of 336 base-pairs of MT ND4, the analysis of intra-specific phylogeny was conducted with the Network Ver. 4.6.10 program; and the phylogenetic analysis, with the Neighbor Joining distance method. The presence of five haplotypes of Ae. Aegypti grouped in two lineages was identified: the first one includes haplotypes 1, 3 and 5, and the second one comprises haplotypes 2 and 4. The geographic distribution of each of the haplotypes found is also shown. It is concluded that this variability is caused by the active migration of this vector and the human activity-mediated passive migration.

Downloads

Download data is not yet available.

Published

2014-02-10

Issue

Section

Research Articles

How to Cite

1.
Yáñez P, Mamani E, Valle J, García MP, León W, Villaseca P, et al. Genetic variability of Aedes aegypti determined by mitochondrial gene ND4 analysis in eleven endemic areas for dengue in Peru. Rev Peru Med Exp Salud Publica [Internet]. 2014 Feb. 10 [cited 2024 Jun. 20];30(2). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/199