Evaluation of minimum thresholds of cellularity in units of umbilical cord blood at Instituto Nacional Materno Perinatal in Peru

Authors

  • Diana Torres Escuela de Tecnología Médica, Universidad Nacional Mayor de San Marcos. Lima, Perú. Instituto de Hemaféresis y Terapia Celular “IHEMATEC”. Lima, Perú. Tecnólogo médico; magíster en Inmunología
  • Willy Cerón Escuela de Tecnología Médica, Universidad Nacional Mayor de San Marcos. Lima, Perú. Universidad Peruana Cayetano Heredia. Lima, Perú. Tecnólogo médico;magíster en Inmunología;
  • Belinda Córdova Escuela de Tecnología Médica, Universidad Nacional Mayor de San Marcos. Lima, Perú. Tecnólogo médico
  • Ricardo Rodríguez Escuela de Tecnología Médica, Universidad Nacional Mayor de San Marcos. Lima, Perú. Tecnólogo médico
  • Efraín Cabrera Escuela de Tecnología Médica, Universidad Nacional Mayor de San Marcos. Lima, Perú. Instituto de Hemaféresis y Terapia Celular “IHEMATEC”. Lima, Perú. Tecnólogo médico
  • Raúl Alegría Escuela de Tecnología Médica, Universidad Nacional Mayor de San Marcos. Lima, Perú. médico
  • José M. Cóndor Escuela de Tecnología Médica, Universidad Nacional Mayor de San Marcos. Lima, Perú. Tecnólogo médico doctor en ciencias

DOI:

https://doi.org/10.17843/rpmesp.2016.334.2553

Keywords:

Blood banks, umbilical cord, fetal blood, antigens, CD34, cord blood stem cell transplantation, birth weight, cryopreservation, Peru

Abstract

A cross-sectional study that included 100 units of umbilical cord blood (UCB) was conducted to evaluate the minimum criteria of cellularity in UCB units, according to NetCord standards at Instituto Nacional Materno Perinatal in Lima, Peru. The volume, total count of nucleated cells by hematological tests and total number of CD34+ as well as cell viability by flow cytometry were determined. The study revealed that 56% of UCB units do not fulfill the minimum criteria of cellularity to be cryopreserved in an umbilical cord blood bank. Furthermore, the UCB units of newborns who weighed more and were female had a higher volume and cell count. In conclusion, these variables must undoubtedly be considered to optimize the collection of UCB units and obtain greater cell counts that enable the storage of high-quality units in a future umbilical cord blood bank in Peru.

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Published

2016-12-13

Issue

Section

Brief Report

How to Cite

1.
Torres D, Cerón W, Córdova B, Rodríguez R, Cabrera E, Alegría R, et al. Evaluation of minimum thresholds of cellularity in units of umbilical cord blood at Instituto Nacional Materno Perinatal in Peru. Rev Peru Med Exp Salud Publica [Internet]. 2016 Dec. 13 [cited 2024 Apr. 17];33(4):695-9. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2553

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