Coccidiosis intestinal en niños admitidos en un hospital de Perú y comparación de dos métodos para la detección del Cryptosporidium spp

Heber Silva-Díaz; Hamer Campos-Flores; Jean Pier Llagas-Linares; Dunalia LLatas-Cancino

doi:10.17843/rpmesp.2016.334.2560

Authors

Heber Silva-Díaz Laboratorio de Parasitología, Metaxénicas y Zoonosis, Hospital Regional Lambayeque. Lambayeque, Perú. Biólogo. doctor en ciencias.
Hamer Campos-Flores Facultad de Ciencias de la Salud, Universidad de Chiclayo. Lambayeque, Perú. tecnólogo médico
Jean Pier Llagas-Linares Facultad de Ciencias de la Salud, Universidad de Chiclayo. Lambayeque, Perú. tecnólogo médico
Dunalia LLatas-Cancino Laboratorio de Parasitología, Metaxénicas y Zoonosis, Hospital Regional Lambayeque. Lambayeque, Perú. maestro en Ciencias

DOI:

https://doi.org/10.17843/rpmesp.2016.334.2560

Keywords:

Intestinal coccidia, Cryptosporidium, Cyclospora, acid-fast stain, enzyme-linked immunosorbent assay

Abstract

A cross-sectional study was done between October 2014 and June 2015 to estimate the frequency of infection due to intestinal coccidiosis in children admitted to a hospital in Peru, and compare the modified acid-fast staining procedure with the enzyme-linked immunoassay (ELISA) method for the detection of Cryptosporidium spp. Coccidia were detected using the modified acid-fast staining procedure and the Cryptosporidium ELISA method (kit r-Biopharm) on seriated stool samples. Out of a total of 325 children, 5.5% had some type of intestinal coccidiosis: 3.7% involved Cryptosporidium spp. (using both techniques) and 1.8% involved Cyclospora cayetanensis (using the modified acid-fast staining procedure). The modified acid-fast staining procedure and ELISA method revealed a 0.955 consistency in the detection of Cryptosporidium spp. In conclusion, intestinal coccidiosis is frequent in children based on the population studied; similarly, both techniques can be used to detect Cryptosporidium spp., but the modified acid-fast staining procedure has an advantage over the other because it is cost-effective and can detect other coccidia in everyday practice.