Rickettsial species in human cases with non-specific acute febrile syndrome in Peru

Authors

  • Rosa Palacios-Salvatierra Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Bióloga, Magister en Salud Pública
  • Omar Cáceres-Rey Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo, Magister en Biología Molecular
  • Andrés Vásquez-Domínguez Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Licenciado en Biología
  • Patricia Mosquera-Visaloth Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Técnica de Laboratorio
  • Elizabeth Anaya-Ramírez Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Bióloga, Magister en Salud Pública

DOI:

https://doi.org/10.17843/rpmesp.2018.354.3646

Keywords:

Rickettsia, Polymerase Chain Reaction, Peru

Abstract

With the objective of molecularly characterizing rickettsial isolates from humans with non-specific acute febrile syndrome, a cross-sectional descriptive study was conducted, with isolates propagated in Vero ATCC cellular cultures and alternative lines, verifying the viability by means of Indirect Immunofluorescence. Prior to DNA extraction, the gltA gene was amplified by means of conventional PCR, and its sequence was analyzed. Twelve isolates were amplified, five with sufficient DNA so as to sequence them, exhibiting compatibility with R. asembonensis in four, and a close identity with Coxiella burnetti in one. At least three of seven alternative cellular lines showed significant yield in sub-cultures. R. asembonensis was identified in four isolates of humans with non-specific acute febrile syndrome, coming from the regions of Ayacucho, Cajamarca, and Madre de Dios in Peru, and Coxiella burnetti in one coming from the Loreto region.

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Published

2018-12-21

Issue

Section

Brief Report

How to Cite

1.
Palacios-Salvatierra R, Cáceres-Rey O, Vásquez-Domínguez A, Mosquera-Visaloth P, Anaya-Ramírez E. Rickettsial species in human cases with non-specific acute febrile syndrome in Peru. Rev Peru Med Exp Salud Publica [Internet]. 2018 Dec. 21 [cited 2024 Nov. 21];35(4):630-5. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/3646

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