Characterization of metallo-β-lactamase in clinical isolates of Pseudomonas aeruginosa retrieved from patients hospitalized in the Central Military Hospital

Authors

  • Gina Salvador-Luján Laboratorio de Microbiología del Hospital Militar Central. Lima, Perú. Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Bióloga con mención en Microbiología y Parasitología, magíster en Microbiología
  • Ruth García-de-la-Guarda Laboratorio de Microbiología Molecular y Biotecnología, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. bióloga microbióloga, magíster en Bioquímica http://orcid.org/0000-0003-4801-5642
  • Edgar Gonzales-Escalante Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales (CITBM), Universidad Nacional Mayor de San Marcos. Lima, Perú. tecnólogo médico, magíster en Microbiología

DOI:

https://doi.org/10.17843/rpmesp.2018.354.3755

Keywords:

Pseudomonas aeruginosa, Carbapenems, Beta-Lactamases, Beta-Lactam resistance, Peru

Abstract

hat codify metallo-β-lactamases (MBL) in clinical isolates of Pseudomona aeruginosa (P. aeruginosa), a cross-sectional study was conducted in the Central Military Hospital (HMC) of Lima, Peru, between January and September, 2016. Seventy-six (76) isolates of P. aeruginosa with diminished sensitivity to carbapenemes and resistant to ceftazidime were analyzed. The phenotype detection of MBL was made by double disc synergy between imipenem and meropenem versus ethylenediaminetetraacetic acid (EDTA), and the identification of the genes was performed by polymerase chain reaction. Of the 76 isolates, 24 (31.6%) were positive for MBL by the phenotype method, genetically confirming all. The blaIMP gene was found in 23/24 (95.8%) and blaVIM in 1/24 (4.2%). No isolate had blaNDM. The blaIMP gene turned out to be the most frequent among clinical isolates of P. aeruginosa not sensitive to carbapenemics at this Hospital.

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Published

2018-12-21

Issue

Section

Brief Report

How to Cite

1.
Salvador-Luján G, García-de-la-Guarda R, Gonzales-Escalante E. Characterization of metallo-β-lactamase in clinical isolates of Pseudomonas aeruginosa retrieved from patients hospitalized in the Central Military Hospital. Rev Peru Med Exp Salud Publica [Internet]. 2018 Dec. 21 [cited 2024 May 28];35(4):636-41. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/3755

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