Caracterización de metalo-β-lactamasas en aislados clínicos de Pseudomonas aeruginosa recuperados de pacientes hospitalizados en el Hospital Militar Central

Gina Salvador-Luján; Ruth García-de-la-Guarda; Edgar Gonzales-Escalante

doi:10.17843/rpmesp.2018.354.3755

Authors

Gina Salvador-Luján Laboratorio de Microbiología del Hospital Militar Central. Lima, Perú. Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Bióloga con mención en Microbiología y Parasitología, magíster en Microbiología
Ruth García-de-la-Guarda Laboratorio de Microbiología Molecular y Biotecnología, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. bióloga microbióloga, magíster en Bioquímica http://orcid.org/0000-0003-4801-5642
Edgar Gonzales-Escalante Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales (CITBM), Universidad Nacional Mayor de San Marcos. Lima, Perú. tecnólogo médico, magíster en Microbiología

DOI:

https://doi.org/10.17843/rpmesp.2018.354.3755

Keywords:

Pseudomonas aeruginosa, Carbapenems, Beta-Lactamases, Beta-Lactam resistance, Peru

Abstract

hat codify metallo-β-lactamases (MBL) in clinical isolates of Pseudomona aeruginosa (P. aeruginosa), a cross-sectional study was conducted in the Central Military Hospital (HMC) of Lima, Peru, between January and September, 2016. Seventy-six (76) isolates of P. aeruginosa with diminished sensitivity to carbapenemes and resistant to ceftazidime were analyzed. The phenotype detection of MBL was made by double disc synergy between imipenem and meropenem versus ethylenediaminetetraacetic acid (EDTA), and the identification of the genes was performed by polymerase chain reaction. Of the 76 isolates, 24 (31.6%) were positive for MBL by the phenotype method, genetically confirming all. The bla_IMP gene was found in 23/24 (95.8%) and bla^VIM in 1/24 (4.2%). No isolate had bla_NDM. The bla^IMP gene turned out to be the most frequent among clinical isolates of P. aeruginosa not sensitive to carbapenemics at this Hospital.