Antimicrobial susceptibility and mutations in the 23S rRNA gen of Helicobacter pylori in dyspeptic patients

Authors

  • Jesús Guzmán Laboratorio Mixto Internacional Andino Amazónico de Química de la Vida, Universidad Peruana Cayetano Heredia. Lima, Perú. Químico farmacéutico, maestro en Bioquímica y Biología Molecular
  • Denis Castillo Laboratorio Mixto Internacional Andino Amazónico de Química de la Vida, Universidad Peruana Cayetano Heredia. Lima, Perú. biólogo, maestro en Bioquímica y Biología Molecular
  • Manuel Ojeda Clínica Médica Cayetano Heredia. Lima, Perú. médico cirujano
  • Michel Sauvain UMR 152 PharmaDev, Université de Toulouse. Toulouse, Francia. químico farmacéutico, PhD en Farmacoquímica http://orcid.org/0000-0003-3919-532X

DOI:

https://doi.org/10.17843/rpmesp.2019.362.3901

Keywords:

Helicobacter infections, Clarithromycin, Microbial sensitivity tests, Microbial drug resistance, Peru

Abstract

In order to evaluate antimicrobial susceptibility and detect specific mutations in the 23S rRNA gene in Helicobacter pylori strains, a cross-sectional study was performed on 95 patients with dyspepsia treated in a private clinic in Lima. Antrum biopsies were collected by endoscopy for isolation and evaluation of antimicrobial susceptibility using the broth microdilution method. The detection of specific mutations was developed by PCR-RFLP. The percentage of infection by Helicobacter pylori was 46.3%. Resistance values of 52.3% to clarithromycin, 29.6% to metronidazole, 45.5% to levofloxacin, and 4.6% to amoxicillin were observed. The percentage of specific A2142G and A2143G mutations associated with clarithromycin resistance was 43.5%. In conclusion, we found that antimicrobial resistance rates and the percentage of Helicobacter pylori strains circulating in a private clinic in Lima were high.

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Published

2019-06-28

Issue

Section

Brief Report

How to Cite

1.
Guzmán J, Castillo D, Ojeda M, Sauvain M. Antimicrobial susceptibility and mutations in the 23S rRNA gen of Helicobacter pylori in dyspeptic patients. Rev Peru Med Exp Salud Publica [Internet]. 2019 Jun. 28 [cited 2024 Jul. 16];36(2):270-4. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/3901