Susceptibilidad antimicrobiana y mutaciones en el gen ARNr 23s de Helicobacter pylori en pacientes dispépticos

Jesús Guzmán; Denis Castillo; Manuel Ojeda; Michel Sauvain

doi:10.17843/rpmesp.2019.362.3901

Authors

Jesús Guzmán Laboratorio Mixto Internacional Andino Amazónico de Química de la Vida, Universidad Peruana Cayetano Heredia. Lima, Perú. Químico farmacéutico, maestro en Bioquímica y Biología Molecular
Denis Castillo Laboratorio Mixto Internacional Andino Amazónico de Química de la Vida, Universidad Peruana Cayetano Heredia. Lima, Perú. biólogo, maestro en Bioquímica y Biología Molecular
Manuel Ojeda Clínica Médica Cayetano Heredia. Lima, Perú. médico cirujano
Michel Sauvain UMR 152 PharmaDev, Université de Toulouse. Toulouse, Francia. químico farmacéutico, PhD en Farmacoquímica http://orcid.org/0000-0003-3919-532X

DOI:

https://doi.org/10.17843/rpmesp.2019.362.3901

Keywords:

Helicobacter infections, Clarithromycin, Microbial sensitivity tests, Microbial drug resistance, Peru

Abstract

In order to evaluate antimicrobial susceptibility and detect specific mutations in the 23S rRNA gene in Helicobacter pylori strains, a cross-sectional study was performed on 95 patients with dyspepsia treated in a private clinic in Lima. Antrum biopsies were collected by endoscopy for isolation and evaluation of antimicrobial susceptibility using the broth microdilution method. The detection of specific mutations was developed by PCR-RFLP. The percentage of infection by Helicobacter pylori was 46.3%. Resistance values of 52.3% to clarithromycin, 29.6% to metronidazole, 45.5% to levofloxacin, and 4.6% to amoxicillin were observed. The percentage of specific A2142G and A2143G mutations associated with clarithromycin resistance was 43.5%. In conclusion, we found that antimicrobial resistance rates and the percentage of Helicobacter pylori strains circulating in a private clinic in Lima were high.