Effects of spironolactone administration on the podocytes loss and progression of experimental diabetic nephropathy

Authors

  • Cristian Aguilar Departamento de Anatomía Patológica, Hospital Nacional Edgardo Rebagliati Martins. Lima, Perú. Médico cirujano
  • Luis Rodríguez-Delfín Laboratorio de Biología Molecular, Facultad de Biología, Universidad Nacional Pedro Ruiz Gallo. Chiclayo, Perú. Laboratorio de Investigación y Diagnóstico en Genética y Biología Molecular GEN MOL. Trujillo, Perú. biólogo doctor en Ciencias con mención en Genética.

DOI:

https://doi.org/10.17843/rpmesp.2012.294.393

Keywords:

Diabetic nephropathies, Podocytes, Fibrosis, Spironolactone, Rats

Abstract

Objectives. Evaluate the effect of spironolactone (SPL) on the loss of podocytes during the progression of experimental diabetic nephropathy (DN). Materials and methods. A group of male Holtzman rats randomly received streptozotocin (diabetic group) or a buffer citrate (control group). Diabetic rats were treated with SPL (50 mg/kg/day). The glomerular area and the cellularity were evaluated by histomorphometric methods. The injury and loss of podocytes was assessed by desmin expression and Wt-1, respectively. The gene expression of TGF-β1 was assessed by RT-PCR. Results. Glucose levels, the glomerular area, the mesangial expansion and collagen content increased significantly in diabetic rats. The administration of SPL prevented these changes without changing glucose levels. Immunostain for Wt-1 decreased significantly while immunostain for desmin increased dramatically in diabetic rats. Treatment with SPL prevented the increase of desmin expression and the loss of Wt-1 expression. Furthermore, the administration of SPL prevented the increase of TGF-β1 mRNA expression in diabetic rats. Conclusions. Treatment with SPL, through independent glucose effects, reduces the loss of podocytes and the progression of DN morphological changes. These results suggest that these effects are mediated, at least in part, by the inhibition of TGF-β1 mRNA expression.

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Published

2014-02-05

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Section

Research Articles

How to Cite

1.
Aguilar C, Rodríguez-Delfín L. Effects of spironolactone administration on the podocytes loss and progression of experimental diabetic nephropathy. Rev Peru Med Exp Salud Publica [Internet]. 2014 Feb. 5 [cited 2024 Mar. 28];29(4). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/393