Multidrug resistance of Salmonella Infantis in Peru: a study through next generation sequencing

Authors

  • Willi Quino Instituto Nacional de Salud. Lima, Perú. Tecnólogo médico. magister en Microbiología. http://orcid.org/0000-0002-3693-1531
  • Carmen Verónica Hurtado Instituto Nacional de Salud, Lima, Perú. biólogo
  • Oscar Escalante-Maldonado Instituto Nacional de Salud, Lima, Perú. biólogo. doctor en Ciencias Médicas http://orcid.org/0000-0002-7139-6271
  • Diana Flores-León Universidad Alas Peruanas. Lima, Perú Instituto Nacional de Salud, Lima, Perú biólogo. magister en Bioquímica, Biología Molecular y Biomedicina.
  • Orson Mestanza Instituto Nacional de Salud, Lima, Perú. biólogo. magister en Bioinformática
  • France Vences-Rosales Instituto Nacional de Salud, Lima, Perú. biólogo.
  • María Luz Zamudio Instituto Nacional de Salud, Lima, Perú. biólogo.
  • Ronnie G. Gavilán Instituto Nacional de Salud, Lima, Perú. biólogo. doctor en Bioquímica y Biología Molecular. http://orcid.org/0000-0003-1437-5607

DOI:

https://doi.org/10.17843/rpmesp.2019.361.3934

Keywords:

Salmonella, Multidrug resistance, Complete genome sequencing

Abstract

Objectives. To describe the phenotypic and genotypic patterns of the antimicrobial resistance of Salmonella Infantis in Peru. Materials and Methods. Two hundred and ninety-seven strains of Salmonella sp. submitted to the National Institute of Health (INS, in Spanish) during 2014-2016 were analyzed. The strains were phenotypically characterized by microbiological, serological, and antimicrobial susceptibility tests. Based on antimicrobial resistance patterns, 46 strains were selected and genetically characterized by next generation sequencing. Results. 193/297 (65%) strains of Salmonella Infantis were identified, of which 143 (74.1%) were multidrug-resistant producers of extended spectrum beta-lactamases (ESBL). The genomic sequencing evidenced a new profile for Salmonella Infantis; additionally, it identified the presence of 15 different genetic determinants of antimicrobial resistance coded in bacterial chromosome and five coded in a megaplasmid. The phenotypic and genotypic resistance patterns matched, with the exception of ceftazidime. Moreover, the 46 strains presented resistance and/or decreased sensitivity to quinolones. Conclusions. Salmonella Infantis has become one of the sero-varieties most frequently referred to the INS, which includes ESBLproducing multidrug-resistant strains with resistance to quinolones. Finally, the relevance of next generation sequencing is reasserted in the characterization of new variants of pathogens that are important for public health, and their potential use in antimicrobial resistance surveillance systems.

Downloads

Download data is not yet available.

Published

2019-03-08

Issue

Section

Original Article

How to Cite

1.
Quino W, Hurtado CV, Escalante-Maldonado O, Flores-León D, Mestanza O, Vences-Rosales F, et al. Multidrug resistance of Salmonella Infantis in Peru: a study through next generation sequencing. Rev Peru Med Exp Salud Publica [Internet]. 2019 Mar. 8 [cited 2024 Apr. 16];36(1):37-45. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/3934

Most read articles by the same author(s)