Development and validation of loop-mediated isothermal amplification for the detection of the Zika virus

Authors

  • Oscar Escalante-Maldonado Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo
  • Ronnie Gustavo Gavilán Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo
  • Maria Paquita García Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. tecnóloga médica
  • Adolfo Marcelo Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo
  • Edson Pacheco Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo
  • César Cabezas Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. médico infectólogo
  • Wataru Yamazaki University of Miyazaki. Miyazaki, Japan. Biólogo

DOI:

https://doi.org/10.17843/rpmesp.2019.363.3941

Keywords:

Zika virus, Diagnosis, RNA-directed DNA polymerase, Polymerase chain reaction

Abstract

A Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) method was developed to detect Zika. The primers were designed based on the NS5 region of 64 complete genomes. Lyophilized LAMP reagent was used. Initially, seven different arboviruses were tested and only Zika samples tested positive. Additionally, serial dilutions of one of Zika's RNA were compared using RT-LAMP and qRT-PCR, demonstrating that RT-LAMP is 1,000 times more sensitive. We also evaluated 300 serum samples with RT-LAMP comparing the results with standard qRT-PCR methods, and we obtained a 99.3% sensitivity, 100% specificity, 100% positive predictive value, and 99.3% negative predictive value. In conclusion, this method provides a low-cost, high-performance, viable, and reliable alternative for the rapid diagnosis of Zika in primary health-care facilities.

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Published

2019-08-13

Issue

Section

Brief Report

How to Cite

1.
Escalante-Maldonado O, Gavilán RG, García MP, Marcelo A, Pacheco E, Cabezas C, et al. Development and validation of loop-mediated isothermal amplification for the detection of the Zika virus. Rev Peru Med Exp Salud Publica [Internet]. 2019 Aug. 13 [cited 2024 Apr. 16];36(3):442-7. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/3941

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