In vitro inhibitory effect of chlorinated tetrasulfonated aluminum phthalocyanine against Leishmania (Viannia) Peruviana and Leishmania (Viannia) Braziliensis

Authors

  • Kelly Vanesa Izarra-Rojas Laboratorio de Ecología Microbiana, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú. Bióloga/o
  • Nyshon Rojas-Palomino Laboratorio de Referencia Nacional de Leishmaniasis, Instituto Nacional de Salud, Lima, Perú. Bióloga/o
  • José Luis Gonzáles-Medrano Laboratorio de Investigación y Desarrollo de Química Orgánica, Universidad Nacional Mayor de San Marcos, Lima – Perú. químico
  • Gloria Minaya-Gómez Laboratorio de Referencia Nacional de Leishmaniasis, Instituto Nacional de Salud, Lima, Perú. bióloga, máster en Medicina Tropical y Salud Internacional
  • Alfredo Berrocal-Huallpa Facultad de Ciencias de la Salud, Universidad Autónoma de Ica, Perú. biólogo, magister en Mejoramiento Genético de Plantas
  • Julio Santiago-Contreras Laboratorio de Investigación y Desarrollo de Química Orgánica, Universidad Nacional Mayor de San Marcos, Lima, Perú. químico, doctor en Química
  • Jorge León-Quispe Laboratorio de Ecología Microbiana, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú. biólogo, magíster en Ciencias Microbiológicas

DOI:

https://doi.org/10.17843/rpmesp.2020.373.4465

Keywords:

Tropical Diseases, Cutaneous Leishmaniasis, Photodynamic Therapy Photosensitizers, Alternative Treatment

Abstract

Objectives: To evaluate the in vitro photodynamic activity of aluminum phthalocyanine tetrasulfonate chloride (AlPc- ClS4) on promastigotes and amastigotes of Leishmania (Viannia) peruviana and Leishmania (Viannia) braziliensis. Materials and methods: The activity of photodynamic therapy using AlPcClS4 on Leishmania promastigote and amastigotes was determined by the Methyl Thiazole Tetrazolium (MTT) colorimetric method and quantitative PCR, respectively. Results: Photodynamic treatment showed an inhibitory effect on promastigotes, particularly on Leishmania (V.) peruviana, to a lesser extent on Leishmania (V.) braziliensis and also on intracellular forms of both species. At 24 hours post-radiation, using concentrations of 200 μM and 350 μM, the inhibitory effect on Leishmania (V.) peruviana was 72.9% and 73.9% respectively; at 96 hours the inhibitory effect was of 78.8% and 80.6%, respectively. Regarding intracellular forms, the inhibitory effect on Leishmania (V.) peruviana amastigotes was 57.8% at 72 hours post-treatment, using a concentration of 200 μM. The IC50 was 56.5, 50, 44 and 39.7 μM, at 24, 48, 72 and 96 hours post-radiation, respectively. Conclusions: Photodynamic therapy using AlPcClS4 against Leishmania species showed encouraging results, mainly on Leishmania (V.) peruviana, suggesting a potential use as an alternative or complement to the usual treatment of tegumentary leishmaniasis. However, new trials are still required to determine the selectivity index for the intracellular form of the parasite, and to develop methods to facilitate the efficient entry of the molecule into the host cell and the parasite.

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Published

2020-07-31

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Section

Original Article

How to Cite

1.
Izarra-Rojas KV, Rojas-Palomino N, Gonzáles-Medrano JL, Minaya-Gómez G, Berrocal-Huallpa A, Santiago-Contreras J, et al. In vitro inhibitory effect of chlorinated tetrasulfonated aluminum phthalocyanine against Leishmania (Viannia) Peruviana and Leishmania (Viannia) Braziliensis. Rev Peru Med Exp Salud Publica [Internet]. 2020 Jul. 31 [cited 2024 Dec. 5];37(3):462-70. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/4465

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