Identificación de especies de Leishmania en pacientes derivados al Instituto Nacional de Salud del Perú

Aidé Sandoval-Juárez; Gloria Minaya-Gómez; Nyshon Rojas-Palomino; Omar Cáceres

doi:10.17843/rpmesp.2020.371.4514

Authors

Aidé Sandoval-Juárez Laboratorio de Referencia Nacional de Leishmaniasis, Instituto Nacional de Salud, Lima, Perú. Bióloga, magister en Salud Pública http://orcid.org/0000-0001-5863-803X
Gloria Minaya-Gómez Laboratorio de Referencia Nacional de Leishmaniasis, Instituto Nacional de Salud, Lima, Perú. bióloga, máster en Medicina Tropical y Salud Internacional http://orcid.org/0000-0002-0097-9568
Nyshon Rojas-Palomino Laboratorio de Referencia Nacional de Leishmaniasis, Instituto Nacional de Salud, Lima, Perú. biólogo http://orcid.org/0000-0003-1303-3744
Omar Cáceres Laboratorio de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima, Perú. Escuela de Medicina Humana, Facultad de Ciencias de la Vida, Universidad Científica del Sur, Lima, Perú. biólogo, magister en Bioquímica y Biología Molecular. http://orcid.org/0000-0003-1529-2475

DOI:

https://doi.org/10.17843/rpmesp.2020.371.4514

Keywords:

Cutaneous Leishmaniasis, kDNA minicircles, Molecular Typing, Real-Time PCR, Peru

Abstract

In Peru, leishmaniasis is a metaxenic disease that represents a serious public health problem, due to its wide distribution and the number of people in danger of contracting the disease, being the vulnerable population mainly those with low economic resources. The study was conducted from patients who were derived to Peru’s National Institute of Health between 2006 and 2011 so that the specialized diagnosis could be carried out. The identification of the species of infectious Leishmania was developed through the analysis of the High-Resolution Melting Analysis obtained from the genomic DNA of promastigotes and amastigotes, which allows to identify the species of Leishmania (Viannia) braziliensis, Leishmania (V.) guyanensis, Leishmania (V.) peruviana as more prevalent, in addition to Leishmania (V.) lainsoni and Leishmania (L.) amazonensis.