Utility of flow citometry for detecting metallo-beta-lactamase-producing Pseudomonas aeruginosa

Authors

  • Iván Alexander Salinas-Salcedo Hospital de Alta Complejidad Virgen de la Puerta, EsSalud, La Libertad, Perú. Universidad Nacional de Trujillo, La Libertad, Perú. Tecnólogo médico magister en Ciencias con mención en Microbiología Clínica
  • José Antonio Saldaña-Jiménez Universidad Nacional de Trujillo, La Libertad, Perú. magister en Biotecnología y Bioenergía doctor en Ciencias Biológicas
  • José Manuel Guevara-Canales Becton Dickinson, Perú Tecnólogo médico
  • Edgar Gonzales-Escalante Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales (CITBM), Universidad Nacional Mayor de San Marcos, Lima, Perú. Laboratorio de Resistencia Bacteriana, Facultad de Farmacia y Bioquímica, Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Universidad de Buenos Aires Argentina. Tecnólogo médico magister en Microbiología

DOI:

https://doi.org/10.17843/rpmesp.2020.374.4825

Keywords:

Pseudomonas aeruginosa, Carbapenems, beta-Lactamases, beta-Lactam resistance, Flow Cytometry

Abstract

In order to determine the utility of flow cytometry for detecting metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa, we used genotypically characterized P. aeruginosa isolates from the Molecular Epidemiology and Genetics Laboratory of the Universidad Nacional Mayor de San Marcos. A total of 29 isolates (17 MBL-producing and 12 non-MBL-producing) were analyzed with the FACSCalibur (Becton Dickinson) cell viability kit. Two treatments were used, one with meropenem and the other with meropenem-EDTA. A significant difference between MBL and non-MBL-producing P. aeruginosa was demonstrated using the fluorescence ratio in non-living cells, considering a cut-off point of >1.6. We determined a sensitivity of 94.1% and a specificity of 100%. Flow cytometry represents an alternative for the detection of MBL-producing P. aeruginosa.

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Published

2020-11-11

Issue

Section

Brief Report

How to Cite

1.
Salinas-Salcedo IA, Saldaña-Jiménez JA, Guevara-Canales JM, Gonzales-Escalante E. Utility of flow citometry for detecting metallo-beta-lactamase-producing Pseudomonas aeruginosa. Rev Peru Med Exp Salud Publica [Internet]. 2020 Nov. 11 [cited 2024 May 25];37(4):700-4. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/4825

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