Study of marine actinomycetes isolated from the central coast of Peru and their antibacterial activity against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecalis

Authors

  • Jorge León Facultad de Ciencias Biológicas – Universidad Nacional Mayor de San Marcos. Lima, Perú. Microbiólogo. Magíster en Ciencias Microbiológicas.
  • Juan José Aponte Facultad de Ciencias Biológicas – Universidad Nacional Mayor de San Marcos. Lima, Perú. Bachiller en Microbiología.
  • Rosario Rojas Laboratorio de Investigación y Desarrollo. Unidad de Investigación en Productos Naturales, Universidad Peruana Cayetano Heredia. Lima, Perú. Médico-Cirujano. PhD en Química Médica y farmacognosia.
  • D´Lourdes Cuadra Facultad de Ciencias Biológicas – Universidad Nacional Mayor de San Marcos. Lima, Perú. Microbiólogo.
  • Nathaly Ayala Facultad de Ciencias Biológicas – Universidad Nacional Mayor de San Marcos. Lima, Perú. Bachiller en Parasitología.
  • Gloria Tomás Laboratorio de Investigaciones de Productos Naturales, Departamento de Química Orgánica, Universidad Nacional Mayor de San Marcos. Lima, Perú. Químico. Magíster en Química.
  • Marco Guerrero Laboratorio de Síntesis Orgánica, Departamento de Química Orgánica, Universidad Nacional Mayor de San Marcos. Lima, Perú. Químico.

DOI:

https://doi.org/10.17843/rpmesp.2011.282.489

Keywords:

Marine sediments, Staphylococcus aureus, Enterococcus faecalis, Antibiosis, Peru

Abstract

Objectives. To determine the antimicrobial potential of marine actinomycetes against drug-resistant pathogens represented by strains of methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecalis (VRE). Materials and methods. Strains of actinomycetes (29) isolated from marine sediment were evaluated by their characteristics in two culture media and by testing their inhibitory capacity by in vitro antagonism against multi-drug resistant (MDR) pathogenic bacteria for MRSA and VRE. Organic extracts of 3 selected actinomicetes were processed to determine the minimum inhibitory concentration (MIC) of the active compound. Results. Most isolated actinomycetes belong to a homogeneous group of write-gray actinomycetes with a good growth in Marine Agar. The inhibitory rates of the isolates were above 85% for both pathogens with inhibition zones greater than 69 and 78 mm in diameter for MRSA and VRE respectively. Dichloromethane extracts of 3 isolates (I-400A, B1-T61, M10-77) showed strong inhibitory activity of both pathogens, M10-77 being the highest actinomycete strain with antibiotic activity against methicillin-resistant S. aureus ATCC 43300 and vancomycin-resistant E. faecalis ATCC 51299 with a minimum inhibitory concentrations (MIC) of 7.9 and 31.7 μg/ml respectively. Phylogenetic analysis of M10-77 strain showed 99% similarity with the marine species Streptomyces erythrogriseus. Conclusions. Marine sediments of the central coast of Peru, are a source of actinomycetes strains showing high capacity to produce bioactive compounds able to inhibit pathogens classified as multi-drugresistant such as methicillin-resistant S. aureus and vancomycin-resistant E. faecalis.

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Published

2011-06-30

Issue

Section

Research Articles

How to Cite

1.
León J, Aponte JJ, Rojas R, Cuadra D, Ayala N, Tomás G, et al. Study of marine actinomycetes isolated from the central coast of Peru and their antibacterial activity against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecalis. Rev Peru Med Exp Salud Publica [Internet]. 2011 Jun. 30 [cited 2024 Jun. 13];28(2). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/489