Identificación de especies de Leishmania en pacientes y flebotominos en áreas de transmisión en una región de Perú

Ofelia Córdova; Franklin Vargas; Yoshihisa Hashiguchi; Hirotomo Kato; Eduardo Gómez

doi:10.17843/rpmesp.2011.283.522

Authors

Ofelia Córdova Universidad Privada Antenor Orrego. La Libertad, Perú. Microbióloga, Doctora en Biotecnología, Postdoctorado en Genética.
Franklin Vargas Instituto de investigación de Microbiología y Parasitología Tropical, Universidad Nacional de Trujillo. La Libertad, Perú. Microbiólogo y Parasitólogo, Doctor en Genética y Biotecnología.
Yoshihisa Hashiguchi Universidad de Kochi. Kochi, Japón. Biólogo.
Hirotomo Kato Universidad de Kochi. Kochi, Japón. Biólogo. Microbiólogo y Parasitólogo, Doctor en Genética y Biotecnología.
Eduardo Gómez Universidad Católica de Guayaquil. Guayaquil, Ecuador. Médico Cirujano.

DOI:

https://doi.org/10.17843/rpmesp.2011.283.522

Keywords:

Leishmaniasis, Endemic Diseases, Cutaneous, Peru

Abstract

Objectives. To identify the species of Leishmania present in the skin lesions of patients and Lutzomyias living in endemic areas of La Libertad, Peru. Materials and methods. Molecular methods based on PCR and RFLP were used, which allowed to have efficient data with small amounts of samples (small specimens), due to their high sensitivity and ease of application in the field work. Results. The results of PCR of clinical samples of patients and insect vectors showed the presence of Leishmania (V.) peruviana as a major causative agent of andean leishmaniasis transmitted by Lutzomyia peruensis. The presence of Leishmania (V.) guyanensis in Lutzomyia ayacuchensis, was found as well. Conclusions. The presence of L. (V.) peruviana and L. (V.) guyanensis in the Andean areas under study was found. These findings remark the need of a wider research about the geographical distribution of L. (V.) guyanensis and clinical features related to the infection in endemic areas of cutaneous leishmaniasis.