Evaluation under field conditions of a rapid test for detection of IgM and igg antibodies against SARS-CoV-2

Authors

  • Margot Vidal-Anzardo Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú. Médico cirujano https://orcid.org/0000-0002-3075-7755
  • Gilmer Solis Oficina General de Investigación y Transferencia Tecnológica, Instituto Nacional de Salud, Lima, Perú. Cirujano dentista https://orcid.org/0000-0001-7084-088X
  • Lely Solari Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú. Médico Infectólogo, doctora en Ciencias de Salud. https://orcid.org/0000-0002-8041-8807
  • Gabriela Minaya Oficina General de Investigación y Transferencia Tecnológica, Instituto Nacional de Salud, Lima, Perú. Médico Infectólogo. https://orcid.org/0000-0002-1193-279X
  • Beatriz Ayala-Quintanilla Oficina General de Investigación y Transferencia Tecnológica, Instituto Nacional de Salud, Lima, Perú. Médico Ginecobstetra, doctora en Medicina. https://orcid.org/0000-0002-2630-4569
  • Jonh Astete-Cornejo Centro Nacional de Salud Ocupacional y Protección del Ambiente para la Salud, Lima, Perú. Médico especialista en Medicina Ocupacional y del Medio Ambiente, magíster en Administración de Empresas con especialidad en Gestión Integrada de la Calidad, Seguridad y Medio Ambiente https://orcid.org/0000-0001-6225-6720
  • Amparo Luque-Aguilar Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú. Médico cirujano; Bióloga https://orcid.org/0000-0003-4087-4469
  • Ana Jorge Laboratorio de Referencia Nacional de Bacterias de Transmisión Sexual, Instituto Nacional de Salud, Lima, Perú. Bióloga https://orcid.org/0000-0003-0570-4640
  • Nancy Rojas Laboratorio de Referencia Nacional de Virus Respiratorio, Instituto Nacional de Salud, Lima, Perú. Bióloga https://orcid.org/0000-0002-3194-8043
  • Fanny Cardenas Laboratorio de Referencia Nacional de Bacterias de Transmisión Sexual, Instituto Nacional de Salud, Lima, Perú. Bióloga https://orcid.org/0000-0002-4541-275X
  • Alonso Soto Instituto de Investigación en Ciencias Biomédicas, Facultad de Medicina Humana. Universidad Ricardo Palma, Lima, Perú. Departamento de Medicina, Hospital Nacional Hipólito Unanue, Lima, Perú. Médico especialista en Medicina Interna y Doctor en Ciencias Médicas https://orcid.org/0000-0001-8648-8032

DOI:

https://doi.org/10.17843/rpmesp.2020.372.5534

Keywords:

Coronavirus, Validation Studies, Serologic Test, SARS-CoV-2, COVID-19

Abstract

Objective. To determine the additional diagnostic performance of a rapid serological test for detection of IgM and IgG antibodies compared to the real-time polymerase chain reaction (RT-PCR) test; for detection of SARS-CoV-2. Methods. A cross-sectional study was carried out including patients hospitalized for COVID-19 in 3 hospitals, health workers exposed to the infection and outpatients who met suspicious case criteria, all of which underwent the molecular test (RT-PCR) and the rapid serological test. The additional diagnostic performance of rapid serological test was evaluated in comparison to molecular tests. Likewise, an approximation was made to the sensitivity and specificity of the rapid serological test. Results. 144 people were included. With the rapid test, 19.4% of positive results were obtained compared to 11.1% in the molecular test (p = 0.03). The rapid serological test detected 21 cases that had been negative by the initial (RT-PCR), providing an additional diagnostic performance of 56.8% compared to the RT-PCR. The additional diagnostic performance was 50.0% during the first week, 70.0% during the second week and 50.0% during the third week of symptom onset. The sensitivity of the rapid serological test was 43.8% and the specificity of 98.9%. Conclusion. The rapid serological test was able to detect a greater number of cases than those detected by the molecular test especially after the second week of onset of symptoms. It also showed high specificity. It is therefore useful as a complementary test to RT-PCR, especially during the second and third week of illness.

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Published

2020-04-22

Issue

Section

Original Article

How to Cite

1.
Vidal-Anzardo M, Solis G, Solari L, Minaya G, Ayala-Quintanilla B, Astete-Cornejo J, et al. Evaluation under field conditions of a rapid test for detection of IgM and igg antibodies against SARS-CoV-2. Rev Peru Med Exp Salud Publica [Internet]. 2020 Apr. 22 [cited 2024 Jun. 15];37(2):203-9. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/5534

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