Characterization of Escherichia coli D7111 producing the β-lactamase TEM-176

Authors

  • Joaquim Ruiz Laboratorio de Genética Molecular y Bioquímica, Universidad Científica del Sur, Lima, Perú. PhD en Biología http://orcid.org/0000-0002-4431-2036
  • Maria J. Pons Laboratorio de Genética Molecular y Bioquímica, Universidad Científica del Sur, Lima, Perú. PhD en Biología http://orcid.org/0000-0001-8384-2315
  • Susan Mosquito Instituto de Medicina Tropical «Alexander von Humboldt», Universidad Peruana Cayetano Heredia, Lima, Perú. PhD en Biología http://orcid.org/0000-0001-9308-1820
  • Theresa J. Ochoa Instituto de Medicina Tropical «Alexander von Humboldt», Universidad Peruana Cayetano Heredia, Lima, Perú. School of Public Health, University of Texas Health Science Center at Houston, Houston, Estados Unidos médico infectóloga pediatra, PhD en Medicina http://orcid.org/0000-0002-3227-3906
  • Yolanda Sáenz Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja, Logroño, España. PhD en Bioquímica y Biología Molecular y Celular http://orcid.org/0000-0002-2457-4258

DOI:

https://doi.org/10.17843/rpmesp.2021.381.6727

Keywords:

Ampicillin, Amoxicillin-Clavulanic Acid, beta-Lactamases, Antibacterial Drug Resistance, Enterotoxigenic Escherichia coli, Molecular Epidemiology, Integrons

Abstract

The present report is the original description of blaTEM-176. The mechanisms of resistance to antimicrobial agents were determined in an enterotoxigenic Escherichia coli, determining the susceptibility to 22 antimicrobials classified in 15 different groups by agar diffusion and establishing the phylogenetic group, mechanisms of resistance and presence of Class 1 and 2 integrons. Integrons and β-lactam resistance genes were sequenced. The isolate, belonging to phylogenetic group A, showed the presence of resistance or diminished susceptibility to a ampicillin, amoxicillin plus clavulanic acid, nalidíxic acid, ciprofloxacin, streptomycin, kanamycin, tetracycline, trimethoprim, sulfisoxazole, cotrimoxazole, azithromycin and nitrofurantoin, carrying blaTEM, aadA1/2, aphA1, sul3, tet(A) and a Class 2 integron containing a dfrA1 gene. Quinolone resistance was related to the substitution Ser83Ala. The TEM sequencing showed the presence of the new substitution Ala222Val, which led to the description of the new β-lactamase blaTEM-176.

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Published

2021-02-03

Issue

Section

Brief Report

How to Cite

1.
Ruiz J, Pons MJ, Mosquito S, Ochoa TJ, Sáenz Y. Characterization of Escherichia coli D7111 producing the β-lactamase TEM-176. Rev Peru Med Exp Salud Publica [Internet]. 2021 Feb. 3 [cited 2024 Jun. 15];38(1):130-5. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/6727

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