Evaluación de dos pruebas de inmunoblot con antígeno hidatídico de caprino y ovino para el diagnóstico de equinococosis humana

Eduardo Miranda; Franko Velarde; Jose Somocurcio; Eduardo Ayala

doi:10.17843/rpmesp.2010.272.1466

Authors

Eduardo Miranda Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo.
Franko Velarde Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo.
Jose Somocurcio Hospital Edgardo Rebagliati Martins, EsSalud. Lima, Perú. Médico Anatomopatólogo.
Eduardo Ayala Hospital Edgardo Rebagliati Martins, EsSalud. Lima, Perú. Médico Anatomopatólogo.

DOI:

https://doi.org/10.17843/rpmesp.2010.272.1466

Keywords:

Echinococcosis, Blotting, western, Sensitivity and specificity, Cross-priming

Abstract

To estimate the diagnosis value of goat and ovine antigen for echinococcosis immunoblot test, 135 serums were used, of which 70 were coming from patients with hydatid disease confirmed by the finding of proto scolex and membrane in the pathology study of surgical piece, 45 from patients with other parasitic diseases and 20 apparently healthy people. The sensitivity, the specificity, positive and negative predictive value of immunoblot test, with hidatyd antigen of goat was of 92.8%, 100%, 100%, 92.8%, respectively, than for ovine antigen was 91.4%, 95.3%, 95.5%, 91.1%, respectively. Kappa index was 0.93 for goat antigen and 0.86 with sheep in relation to the pathological study. We recommended the use of both antigens for the serologic diagnosis of human echinococcosis.