Intervalos de referencia de subpoblaciones linfocitarias de sangre periférica en adultos sanos de Lima, Perú

José M. Cóndor; Marco Álvarez; Luis Cano; Edgar Matos; Christian Leiva; José A. Paredes

doi:10.17843/rpmesp.2013.302.197

Authors

José M. Cóndor Instituto de Hemaferesis y Terapia Celular “IHEMATEC”. Lima, Perú. Facultad de Medicina, Universidad Nacional Mayor de San Marcos. Lima, Perú. Tecnólogo médico.
Marco Álvarez Instituto de Hemaferesis y Terapia Celular “IHEMATEC”. Lima, Perú. Tecnólogo médico.
Luis Cano Instituto de Hemaferesis y Terapia Celular “IHEMATEC”. Lima, Perú. Facultad de Medicina, Universidad Nacional Mayor de San Marcos. Lima, Perú. Tecnólogo médico.
Edgar Matos Instituto de Hemaferesis y Terapia Celular “IHEMATEC”. Lima, Perú. médico inmunólogo y alergólogo.
Christian Leiva Rochem Biocare. Bogotá, Colombia. Tecnólogo médico.
José A. Paredes Facultad de Medicina, Universidad Nacional Mayor de San Marcos. Lima, Perú. Tecnólogo médico. magister en Medicina Transfusional.

DOI:

https://doi.org/10.17843/rpmesp.2013.302.197

Keywords:

Lymphocyte subsets, Flow cytometry, Reference values, Peru

Abstract

In order to establish the reference intervals (RIs) of peripheral blood lymphocyte subsets (PBL) in healthy adults in Lima (Peru), a cross-sectional study was conducted among blood donors taken in between 2011 and 2012. Based on the criteria obtained from the guidelines of the Clinical and Laboratory Standards Institute (CLSI C28-A3), 318 samples were processed, 61.9% (197/318) coming from male donors. For PBL count, a flow cytometer with a simple platform was used. The RIs are established for each PBL in adults based on sex with their respective reference limits and 90% confidence intervals. Differences were found in CD3+ percentage counts (p=0.001) and in CD3-CD56+ absolute (p=0.003) and percentage counts (p˂0.001). The RIs found are different to those described in studies conducted in other countries due to the characteristics of the population and the study model.