Differential expression of circulating micro-RNAs in patients with active tuberculosis and infected with latent tuberculosis
DOI:
https://doi.org/10.17843/rpmesp.2020.371.4468Keywords:
Latent Tuberculosis, Tuberculosis, microRNAs, PeruAbstract
Objectives: To analyze the differential expression of miR-21, miR-29a, miR-99b and miR-155 in serum samples from patients with latent tuberculosis (TB) and active TB compared to healthy controls. Materials and Methods: We used 28 serum samples (9 with active TB, 10 with latent TB and 9 healthy controls) for the analysis of gene expression by RT-qPCR with Primers and TaqMan probes. The differential expression was calculated by the Livak method using a normalizing gene (RNU-48). Results: Overexpression of miR-155 was found in people with latent tuberculosis, compared to healthy controls (0.63 vs. 0.01; p value = 0.032). Conclusion: The miR-155 could be considered a biomarker to differentiate latent TB from active disease. Studies with larger sample sizes are required to corroborate the findings.Downloads
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Published
2020-03-23
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Original Article
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Copyright (c) 2020 Revista Peruana de Medicina Experimental y Salud Pública
This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
1.
Yareta J, Galarza M, Capristano S, Pellón O, Sánchez C, Ballon J, et al. Differential expression of circulating micro-RNAs in patients with active tuberculosis and infected with latent tuberculosis. Rev Peru Med Exp Salud Publica [Internet]. 2020 Mar. 23 [cited 2024 Nov. 24];37(1):51-6. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/4468
