Determinación de la resistencia a antibióticos mediante tres métodos fenotípicos en cepas de Campylobacter coli aisladas de carne de pollo comercializada en Lima, Perú
DOI:
https://doi.org/10.17843/rpmesp.2025.422.14330Palabras clave:
Resistencia a Antibióticos, Campylobacter coli, carne, pollo, Concentración Inhibitoria MínimaResumen
Objetivos. Determinar la resistencia y concentración inhibitoria mínima (CIM) de eritromicina, azitromicina, ciprofloxacina y tetraciclina en cepas de Campylobacter coli aisladas de carcasas de pollos comercializados en Lima, Perú. Materiales y métodos. Cepas de C. coli (n=106) criopreservadas se reactivaron y se evaluó la concordancia (Coeficiente
Kappa) de los resultados de resistencia y CIM entre las pruebas de difusión en disco (DD), E-test (ET) y microdilución en placa (MDP). Resultados. Se reactivaron 97 cepas de las cuales entre 94 al 100% fueron resistentes a ciprofloxacina, eritromicina y tetraciclina; mientras que solo el 58% fue a azitromicina en la prueba de DD. Las pruebas de ET y MDP evidenciaron entre 78 al 100% de cepas resistentes, siendo la azitromicina que presentó el menor porcentaje de resistencia. Más del 70% de cepas presentaron resistencia a por lo menos tres antibióticos en las tres pruebas. Además, el 50%, 69% y 100% de cepas presentaron una CIM ≥ 32 μg/mL para ciprofloxacina, azitromicina y tetraciclina/eritromicina,
respectivamente. Conclusiones. Las cepas de C. coli provenientes de carcasas de pollos tuvieron un alto porcentaje de multidrogorresistencia. La concordancia entre las tres pruebas fue casi perfecta, pero las tiras del ET presentaron concentraciones máximas que son insuficientes para la CIM en estas cepas. Es recomendable realizar la evaluación de la resistencia y la CIM mediante la MDP ya que permite utilizar un mayor rango de concentraciones de los antibióticos.
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Derechos de autor 2025 Kiara N. Cáceres-Bautista, Jorge L. Arroyo-Acevedo, Hugo J. Justil-Guerrero, Johnny A. Tinco-Jayo, Edwin C. Enciso-Roca, Enrique J. Aguilar-Felices, Miguel A. Rojas-Montes, Diego Diaz-Coahila, César A. Lázaro-de la Torre
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
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