Biochemicals characteristics and pre-clinical testing of lyophilized bothropic antivenom against bothrops atrox snake venom

Authors

  • Patricia J. García Centro Nacional de Productos Biológicos, Instituto Nacional de Salud. Lima, Perú. Médico.
  • Armando Yarlequé Laboratorio de Biología Molecular. Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Biólogo.
  • Cesar Bonilla-Ferreyra Centro Nacional de Productos Biológicos, Instituto Nacional de Salud. Lima, Perú. Biólogo.
  • Silvia Pessah Centro Nacional de Productos Biológicos, Instituto Nacional de Salud. Lima, Perú. Médico.
  • Dan Vivas Laboratorio de Biología Molecular. Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Biólogo.
  • Gustavo Adolfo Sandoval Laboratorio de Biología Molecular. Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Biólogo.
  • Fanny Lazo Laboratorio de Biología Molecular. Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Biólogo.

DOI:

https://doi.org/10.17843/rpmesp.2008.254.1302

Keywords:

Antivenins, Snake venoms, Bothrops, Freeze drying

Abstract

Biochemical features and neutralizing capacity of lyophilized bothropic antivenom elaborated by the Peruvian National Health Institute (Lima, Peru). It was found that the antivenom protein contents is 51.4 mg/mL. Lyophilized preparations can be reconstituted in 10 minutes, reaching Abs600nm and pH values reported as 0.091 and 7.0, respectively. Regarding toxicity of the venom for mice, LD50 was 3.33 µg, minimal hemorrhagic dose was 4.10 ± 0.64 µg, minimal myotoxic dose was 30.2 ± 2.5 µg, minimal coagulant dose was 4.50 ± 0.6 µg, and the minimal defibrinating dose was 8 µg; and the effective dose values of the antivenom for the aforementioned parameters were 140.48 (120.09-164.33), 230.67 ± 11.78, 316.56 ± 40.31, 105.5 ± 4.2, and 500 µL antivenom/mg venom, respectively, indicating that this preparation has the ability to neutralize each of the parameters tested. For these reasons we conclude that the investigated product complies with the World Health Organization (WHO) requirements to be considered an effective antivenom capable of neutralizing the main biological activities previously mentioned.

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Published

2008-12-30

Issue

Section

Research Articles

How to Cite

1.
García PJ, Yarlequé A, Bonilla-Ferreyra C, Pessah S, Vivas D, Sandoval GA, et al. Biochemicals characteristics and pre-clinical testing of lyophilized bothropic antivenom against bothrops atrox snake venom. Rev Peru Med Exp Salud Publica [Internet]. 2008 Dec. 30 [cited 2024 Jun. 22];25(4). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/1302

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