Adhesion patterns in diffusely adherent Escherichia coli (DAEC) strains isolated from children with and without diarrhea

Authors

  • Maribel Riveros Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima, Perú. Biólogo.
  • Francesca Barletta Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima, Perú. Magíster en Biología Molecular y Bioquímica.
  • Martín Cabello Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima, Perú. Tecnólogo Médica.
  • David Durand Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima, Perú. Biólogo.
  • Erik H. Mercado Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima, Perú. Biólogo.
  • Carmen Contreras Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima, Perú. Magíster en Biología Molecular y Bioquímica.
  • Fulton P. Rivera Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima, Perú. Tecnólogo Médica.
  • Susan Mosquito Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima, Perú. Magíster en Biología Molecular y Bioquímica.
  • Ángela Lluque Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima, Perú. biólogo.
  • Theresa J. Ochoa Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima, Perú. University of Texas School of Public Health, Houston, Texas, Estados Unidos. Médica Infectóloga Pediatra.

DOI:

https://doi.org/10.17843/rpmesp.2011.281.451

Keywords:

Diffusely Adherent Escherichia coli, Diarrhea, Child, Bacterial Adhesion

Abstract

Introduction. Diffusely adherent E. coli (DAEC) is the sixth recognized group of diarrheagenic E. coli. However, its association with diarrhea remains controversial. Variability in the adherence patterns of clinical strains is unknown. Objectives. To compare the adherence patterns between strains isolated from children with and without diarrhea. Materials and methods. A total of 31 DAEC strains were analyzed, 25 from children with diarrhea and 6 from asymptomatic (control) children, isolated from a cohort study of children under one year of age in the southern districts of Lima. DAEC were identified by PCR (daaD gene). The pattern and adherence score in HEp-2 cell culture were evaluated, Actin polimerization was determined by fluorescence actin staining (FAS) and motility was evaluated by conventional microbiology methods. Results. Diffuse adherence pattern was found in 88% of diarrhea samples and in the total of control strains. The number of bacteria adhered per cell was significantly lower in diarrhea samples (p<0.05). However, actin polymerization was greater in diarrhea samples (60% vs. 17%). Motility test was positive in 60% of the diarrhea samples and in all control samples. Conclusions. Our findings suggest a difference between adherence patterns, actin polymerization and motility between DAEC strains corresponding to diarrhea and control groups. The significance of these results must be confirmed with a bigger number of strains and determining the presence of virulence genes in the strains.

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Published

2011-03-31

Issue

Section

Research Articles

How to Cite

1.
Riveros M, Barletta F, Cabello M, Durand D, Mercado EH, Contreras C, et al. Adhesion patterns in diffusely adherent Escherichia coli (DAEC) strains isolated from children with and without diarrhea. Rev Peru Med Exp Salud Publica [Internet]. 2011 Mar. 31 [cited 2024 Mar. 28];28(1). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/451

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