Mutant alleles associated to chloroquine and sulfadoxine-pyrimethanime resistance in Plasmodium falciparum of the Ecuador-Peru and Ecuador-Colombia borders

Authors

  • Nancy Arróspide Centro Nacional de Salud Pública. Instituto Nacional de Salud. Lima, Perú. Biólogo
  • Gisely Hijar-Guerra Centro Nacional de Salud Pública. Instituto Nacional de Salud. Lima, Perú. Biólogo
  • Doménica de Mora Sistema Nacional de Control de Enfermedades Transmitidas por Vectores Artrópodos. Quito, Ecuador. Biólogo.
  • César Eduardo Diaz-Cortéz Sistema Nacional de Control de Enfermedades Transmitidas por Vectores Artrópodos. Quito, Ecuador. Médico salubrista.
  • Raúl Veloz-Perez Sistema Nacional de Control de Enfermedades Transmitidas por Vectores Artrópodos. Quito, Ecuador. Médico epidemiólogo.
  • Sonia Gutierrez Centro Nacional de Salud Pública. Instituto Nacional de Salud. Lima, Perú. Biólogo.
  • César Cabezas _Sánchez Centro Nacional de Salud Pública. Instituto Nacional de Salud. Lima, Perú. Médico infectólogo.

DOI:

https://doi.org/10.17843/rpmesp.2014.312.47

Keywords:

Plasmodium falciparum, Drug resistance, bacterial, Chloroquine, Sulfadoxine, Pyrimethamine

Abstract

The frequency of mutations in pfCRT and DHFR/DHPS genes of Plasmodium falciparum associated with resistance to chloroquine and sulfadoxine-pyrimethamine was evaluated in 83 strains from the districts of Esmeralda and Machala, located on the borders of Ecuador-Peru and Ecuador-Colombia in 2002. Polymerase chain reaction (PCR), conventional and its variants, was used. Mutations in the pfCRT gene were found in more than 90% of the samples from Esmeralda and Machala. For the DHFR gene, 90% of the strains were mutant samples from Esmeralda, 3 were double mutations and 1 was a triple mutation. In Machala, 25% were simple mutant forms and 75% mixed mutant forms (wild forms/mutant). In conclusion, resistance to chloroquine has been fixed in strains carrying K76T pfCRT mutation, whereas genetic imprinting for resistance to pyrimethamine is evolving, particularly in the district of Esmeralda.

Downloads

Download data is not yet available.

Published

2014-07-02

Issue

Section

Research Articles

How to Cite

1.
Arróspide N, Hijar-Guerra G, de Mora D, Diaz-Cortéz CE, Veloz-Perez R, Gutierrez S, et al. Mutant alleles associated to chloroquine and sulfadoxine-pyrimethanime resistance in Plasmodium falciparum of the Ecuador-Peru and Ecuador-Colombia borders. Rev Peru Med Exp Salud Publica [Internet]. 2014 Jul. 2 [cited 2024 Mar. 28];31(2). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/47