Frecuencia de genes que codifican proteínas autotransportadoras serinproteasa de Enterobacteriaceae (SPATE) en cepas de Escherichia coli difusamente adherente (DAEC) provenientes de niños con y sin diarrea

Karen Ocampo; Maribel Riveros; Maria Pinedo-Bardales; Joaquim Ruiz; Theresa J. Ochoa

doi:10.17843/rpmesp.2021.381.5750

Authors

Karen Ocampo Laboratorio de Infectología Pediátrica, Instituto de Medicina Tropical «Alexander von Humboldt», Universidad Peruana Cayetano Heredia, Lima, Perú.Universidad Nacional Federico Villarreal, Lima, Perú.Bióloga https://orcid.org/0000-0001-7543-9307
Maribel Riveros Laboratorio de Infectología Pediátrica, Instituto de Medicina Tropical «Alexander von Humboldt», Universidad Peruana Cayetano Heredia, Lima, Perú.Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima, Perúmagíster en Biología Molecular https://orcid.org/0000-0002-5347-3261
Maria Pinedo-Bardales Laboratorio de Infectología Pediátrica, Instituto de Medicina Tropical «Alexander von Humboldt», Universidad Peruana Cayetano Heredia, Lima, Perú.Facultad de Ciencias, Universidad Nacional de Piura, Piura, Perú.estudiante de Biología https://orcid.org/0000-0002-0169-5620
Joaquim Ruiz ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, España. Laboratorio de Microbiología Molecular y Genómica Bacteriana, Universidad Científica del Sur, Lima, Perú. microbiólogo, doctor en Biología https://orcid.org/0000-0002-4431-2036
Theresa J. Ochoa Laboratorio de Infectología Pediátrica, Instituto de Medicina Tropical «Alexander von Humboldt», Universidad Peruana Cayetano Heredia, Lima, Perú. School of Public Health, University of Texas Health Science Center at Houston, Houston, Estados Unidos. médica especialista en Infectología Pediátrica https://orcid.org/0000-0002-3227-3906

DOI:

https://doi.org/10.17843/rpmesp.2021.381.5750

Keywords:

Diffusely Adherent Escherichia coli, Virulence Factors, Diarrhea, Child

Abstract

The aim of the study was to determine the frequency of six genes encoding serine protease autotransporter proteins Enterobacteriaceae (SPATE) in diffusely adherent Escherichia coli (DAEC) isolates from children with (WD, n=63) and without diarrhea (WOD, n=41) from Lima, Peru. WOD were considered a control group. For the detection of the genes, 2 multiple PCRs were standardized: triple A (sigA, pet, espP) and triple B (sat, pic, espC). In both groups, the most frequent SPATE gene was Sat (39.7% of WD and 41.5% of WOD), followed by spP (20.6% and 9.7% in WD and WOD respectively). The other genes were detected in proportions lower than 10.0%, in the following order of frequency: pet, sigA, espC and pic, without significant differences between the groups. It was concluded that Sat is the most frequent SPATE in DAEC and that these strains may possess SPATE genes regardless of whether they are isolated in WD or WOD.