Molecular characterization of virulence (lmb, bca and rib) and macrolid resistance genes (ermB, ermTR and mefA) in clinical isolates of Streptococcus agalactiae

Authors

  • Angie Pulido-Colina Universidad Nacional Federico Villarreal, Lima, Perú. Universidad Nacional Mayor de San Marcos, Lima, Perú. Tecnólogo médico https://orcid.org/0000-0002-7619-4353
  • Javier Soto Pastrana Hospital Nacional Docente Madre-Niño San Bartolomé, Lima, Perú. Tecnólogo médico https://orcid.org/0000-0001-9534-5746
  • Ester Valencia-Bazalar Instituto de Medicina Tropical «Daniel A. Carrión», Departamento Académico de Microbiología Médica. Facultad de Medicina. Universidad Nacional Mayor de San Marcos, Lima, Perú. Tecnólogo médico https://orcid.org/0000-0002-4059-9842
  • Milagros Zavaleta Apestegui Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales, Universidad Nacional Mayor de San Marcos, Lima, Perú. biólogo molecular https://orcid.org/0000-0002-2743-9035

DOI:

https://doi.org/10.17843/rpmesp.2021.384.8726

Keywords:

Streptococcus agalactiae, PCR, Genes, Virulence, Drug Resistance, Macrolides, Microbial Sensitivity Test

Abstract

The aim of the study was to molecularly identify virulence and macrolide resistance genes in clinical isolates of Streptococcus agalactiae (GBS), recovered in 2019 from vaginal discharge (n=9) and urine (n=22), from two health facilities in Lima. Identification and antimicrobial susceptibility were determined by the Vitek® 2 automated system, identification was confirmed phenotypically; macrolide resistance was determined by the D-test method. Identification of virulence genes (lmb, bca and rib) and macrolide resistance genes (ermB, ermTR and mefA) was carried out by polymerase chain reaction (PCR). The predominant macrolide resistance phenotype and genotype were cMLSb (12/31) and ermB (11/31); the most frequent virulence gene was lmb (23/31). All were sensitive to penicillin, ampicillin and vancomycin. These findings show the need to implement molecular epidemiology studies that allow adequate knowledge and follow-up of GBS in Peru.

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Published

2022-04-04

Issue

Section

Brief Report

How to Cite

1.
Pulido-Colina A, Soto Pastrana J, Valencia-Bazalar E, Zavaleta Apestegui M. Molecular characterization of virulence (lmb, bca and rib) and macrolid resistance genes (ermB, ermTR and mefA) in clinical isolates of Streptococcus agalactiae. Rev Peru Med Exp Salud Publica [Internet]. 2022 Apr. 4 [cited 2024 Mar. 29];38(4):615-20. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/8726

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