Actividad antiinflamatoria in vitro de Plantago major L. y Piper aduncum L. sobre la fosfolipasa A2 del veneno de la serpiente Lachesis muta muta

Mirtha Yarleque-Chocas; Flor Dorregaray-Llerena; Armando Yarleque-Chocas; Celso Gonzales-Chavesta

doi:10.17843/rpmesp.2023.403.12191

Authors

Mirtha Yarleque-Chocas Laboratorio de Investigación de Bioquímica y Principios Activos Naturales, Facultad de Medicina Hipólito Unanue, Universidad Nacional Federico Villarreal, Lima, Perú. https://orcid.org/0000-0002-3440-1780
Flor Dorregaray-Llerena Laboratorio de Recursos Fitogenéticos, Facultad de Ciencias Agrarias, Universidad Agraria del Ecuador, Milagro, Ecuador. https://orcid.org/0000-0002-8737-7032
Armando Yarleque-Chocas Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú. https://orcid.org/0000-0002-8038-2162
Celso Gonzales-Chavesta Departamento de Estadística e Informática, Universidad Nacional Agraria la Molina, Lima, Perú. https://orcid.org/0000-0001-6420-2443

DOI:

https://doi.org/10.17843/rpmesp.2023.403.12191

Keywords:

Plant extracts, Plantago, Piper, Venom, Phospholipase A2, Anti-inflammatory

Abstract

Objective. To evaluate the in vitro inhibitory activity of Plantago major “llantén” and Piper aduncum “matico” extracts on phospholipase A2 (PLA2) from the venom of the snake Lachesis muta muta. Materials and methods. We carried out an explanatory study with experimental design. Leaves of P. major and P. aduncum were collected in the province of Huarochirí in Lima, Peru. Then, we prepared alcoholic extracts diluted in distilled water and conducted phytochemical assays, quantification of phenols and
flavonoids, thin layer chromatography (TLC) on cellulose and enzymatic activity with PLA2. The ability to inhibit PLA2 with the extracts under study and their fractions was analyzed. The Kruskal Wallis test and Bonferroni multiple comparisons were used during statistical analysis. Results. Phenols, flavonoids and tannins were qualitatively identified in both P. major and P. aduncum; in addition, P. aduncum presented saponins. The inhibition of PLA2 activity of the venom by the total extract of P. major was 45.3%, and its fractions showed the following inhibition values: 31.1% for LLF-1, 66.3% for LLF-2 and 65.5% for LLF-3. The inhibition values for the total extract of P. aduncum were 86.9%, and its fractions showed the following inhibition rates: 34.3% for MF-1, 67.1% for MF-2 and 54.9% for MF-3. Statistical analysis
showed significant differences in the inhibition of PLA2 (p=0.009) by the extracts. Conclusion. The tests demonstrated an association between the anti-Inflammatory effect of the extracts and PLA2 inhibition.

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