Genotipos de virulencia de Helicobacter pylori y su asociación con lesiones precursoras de malignidad gástrica y parámetros histológicos en pacientes colombianos

Claudia Acosta-Astaiza; Alexis López-Sandoval; Juan Bonilla-Chaves; Anyi Valdes-Valdes; William Romo-Romero

doi:10.17843/rpmesp.2023.403.12858

Authors

Claudia Acosta-Astaiza Grupo de Investigación en Genética Humana Aplicada, Universidad del Cauca, Popayán, Colombia https://orcid.org/0000-0001-9703-5653
Alexis López-Sandoval Grupo de Investigación en Genética Humana Aplicada, Universidad del Cauca, Popayán, Colombia https://orcid.org/0000-0002-7709-4047
Juan Bonilla-Chaves Grupo de Investigación en Genética Humana Aplicada, Universidad del Cauca, Popayán, Colombia https://orcid.org/0000-0003-0549-9296
Anyi Valdes-Valdes Grupo de Investigación en Genética Humana Aplicada, Universidad del Cauca, Popayán, Colombia https://orcid.org/0000-0003-2713-4888
William Romo-Romero Hospital Susana López de Valencia, Popayán, Colombia https://orcid.org/0000-0001-9917-0277

DOI:

https://doi.org/10.17843/rpmesp.2023.403.12858

Keywords:

Helicobacter pylori, Gastritis, Bacterial Adhesins, Virulence Factors, Inflammation

Abstract

The aim of this research was to determine the presence of Helicobacter pylori virulence genotypes and their association with precursor lesions of gastric malignancy and histological parameters in patients with dyspepsia
symptoms in southwestern Colombia. Polymerase chain reaction (PCR) was used for the genetic characterization
of vacA, cagA, babA2 and sabA. The chi-square or Fischer test were used to evaluate the association between each genotype and the clinical outcome. We found that 86.3% of the patients with precursor lesions of gastric malignancy presented the vacA s1/m1 genotype, 68.1% had the cagA+ genotype and 68.8% and 55.8% had the babA2+ and sabA+ genotypes, respectively. Our results show association between virulence genotypes and severe degree of polymorphonuclear cell infiltration. In addition, we found an association between the combination of vacA/cagA, vacA/sabA and babA2/sabA genes. This study provides evidence about the association of H. pylori virulence genotypes and gastric inflammation in infected patients.

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