Diversidad clonal de Pseudomonas aeruginosa productoras de carbapenemasas aisladas de muestras clínicas en un hospital de tercer nivel de Perú

Gina Nilda Salvador Lujan; Liz Erika Cruz Pio; Hedersson Calla; Damaris Rivera Ascencios; Luis Solís Cayo; Ruth García de la Guarda

doi:10.17843/rpmesp.2025.421.13818

Authors

Gina Salvador-Lujan Laboratorio de Microbiología del Hospital Militar Central «Crl. Luis Arias Schreiber», Lima, Perú. Laboratorio de Ecología Microbiana, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú. https://orcid.org/0000-0001-6617-6402
Liz Erika Cruz-Pio Laboratorio de Microbiología Molecular y Biotecnología, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú https://orcid.org/0000-0002-8226-7440
Hedersson Calla Laboratorio de Microbiología Molecular y Biotecnología, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú https://orcid.org/0000-0003-3118-1222
Damaris Rivera-Asencios Laboratorio de Microbiología Molecular y Biotecnología, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú https://orcid.org/0000-0002-3462-1791
Luis Solís-Cayo Laboratorio de Microbiología Molecular y Biotecnología, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú https://orcid.org/0000-0002-3240-5323
Ruth García-de-la-Guarda Laboratorio de Microbiología Molecular y Biotecnología, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú https://orcid.org/0000-0003-4801-5642

DOI:

https://doi.org/10.17843/rpmesp.2025.421.13818

Keywords:

Pseudomonas aeruginosa, Metallo-β-lactamases, Molecular Typing, Carbapenemases

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen associated with health care infections, it has high levels of antimicrobial resistance and is associated with hospital outbreaks. Early outbreak detection is a usual problem in hospitals, therefore, this study aimed to assess the clonal relationship of carbapenemaseproducing P. aeruginosa in a tertiary hospital in Lima, Peru. Twenty-four metallo β-lactamase-producing P. aeruginosa strains isolated from hospitalized patients were collected. The clonal relation was determined using the REP-PCR technique. REP-PCR band profiles were normalized, analyzed and combined using BioNumerics version 7.6 software. Molecular identification showed 19 different profiles and four clonal groups. We determined polyclonality among isolates. We did not find clonal dissemination among the
metallo-β-lactamase-producing P. aeruginosa strains circulating in the hospital.

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