PCR-RFLP identification of bacteria present in colostrum of women from the city of Barranquilla, Colombia
DOI:
https://doi.org/10.17843/rpmesp.2025.422.14321Keywords:
Colostrum, Polymorphism, Restriction Fragment Length, Microbiota, Breast FeedingAbstract
Bacteria present in the colostrum of women from Barranquilla city in Colombian Caribbean, were isolated and identified. This descriptive study involved cultivating 55 colostrum samples on M17, MRS, and TOS agars, followed by incubation under both aerobic and anaerobic conditions. A total of 350 microorganism isolates were obtained, of which 296 were identified at the genus level using PCR-RFLP with Hae III and RsaI enzymes. The in-silico identification of the isolates was performed using the online program kodebio. shinyapps.io/RFLP-inator. Seven hypothetical bacterial genres were identified: Staphylococcus, Lactobacillus, Enterococcus, Bifidobacterium, Corynebacterium, Streptococcus, and Leuconostoc. The dominant genera, Staphylococcus, Lactobacillus, and Enterococcus, accounted for 61.1% of the identified microorganisms. The findings of this research provide a starting point in knowledge of the microbial composition of human colostrum, considering the specific context of the environmental conditions of the Colombian Caribbean”.
Downloads
References
Zimmermann P, Curtis N. Breast milk microbiota: A review of the factors that influence composition. J Infect. 2020;81(1):17-47. doi:
1016/j.jinf.2020.01.023.
El-Loly M. Colostrum ingredients, its nutritional and health benefits-an overview. Clin Nutr Open Sci. 2022;44:126-143. doi: 10.1016/j.nutos.2022.07.001.
Fernández L, Pannaraj PS, Rautava S, Rodríguez JM. The microbiota of the human mammary ecosystem. Front Cell Infect Microbiol.
;10:586667. doi: 10.3389/fcimb.2020.586667.
Lyons KE, Ryan CA, Dempsey EM, Ross RP, Stanton C. Breast milk, a source of beneficial microbes and associated benefits for infant health. Nutrients. 2020;12(4):1039. doi: 10.3390/nu12041039.
Lugli GA, Milani C, Turroni F, Tremblay D, Ferrario C, Mancabelli L, et al. Prophages of the genus Bifidobacterium as modulating agents of the infant gut microbiota. Environ Microbiol. 2016;18:2196–2213. doi: 10.1111/1462-2920.13154.
Granger CL, Embleton ND, Palmer JM, Lamb CA, Berrington JE, Stewart CJ. Maternal breastmilk, infant gut microbiome and the impact
on preterm infant health. Acta Paediatri. 2021;110(2):450-457. doi: 10.1111/apa.15534.
Moossavi S, Sepehri S, Robertson B, Bode L, Goruk S, Field CJ, et al. Composition and variation of the human milk microbiota are influenced by maternal and early-life factors. Cell Host Microbe. 2019;25:324–335. e4. doi: 10.1016/j.chom.2019.01.011.
Toscano M, De Grandi R, Peroni DG, Grossi E, Facchin V, Comberiati P, Drago L. Impact of delivery mode on the colostrum microbiota composition. BMC Microbiology. 2017;17:205. doi: 10.1186/s12866-017-1109-0.
Lee H, Lee CK, Kim K. Isolation of Novel Strains of Lactobacillus gasseri EJL and Bifidobacterium breve JTL from Breast Milk and Infant Feces: A Longitudinal Study of a Mother-infant Pair. Microbiol Biotechnol Lett. 2021; 49(1): 1-8. doi: 10.48022/mbl.2010.10011.
Hunt KM, Foster JA, Forney LJ, Schütte UME, Beck DL, Abdo Z, et. al. Characterization of the diversity and temporal stability of bacterial communities in human milk. PLoS One 2011;6:1–8. doi: 10.1371/journal.pone.0021313.
Sakwinska O, Moine D, Delley M, Combremont S, Rezzonico E, Descombes P, Thakkar SK. Microbiota in breast milk of Chinese lactating mothers. PLoS One. 2016;11(8):e0160856. doi: 10.1371/journal.pone.0160856.
Fitzstevens JL, Smith KC, Hagadorn JI, Caimano MJ, Matson AP, Brownell EA. Systematic review of the human milk microbiota. Nutr Clin Pract. 2017;32(3):354-364. doi: 10.1177/0884533616670150.
Jiménez E, Delgado S, Fernández L, García N, Albújar M, Gómez A, Rodríguez JM. Assessment of the bacterial diversity of human colostrum and screening of staphylococcal and enterococcal populations for potential virulence factors. Microbiol Res. 2008:159(9-10):595-601. doi: 10.1016/j.resmic.2008.09.001.
Gámez-Valdez JS, García-Mazcorro JF, Montoya-Rincón AH, Rodríguez-Reyes DL, Jiménez-Blanco G, Rodríguez MA, Licona-Cassani C.
Differential analysis of the bacterial community in colostrum samples from women with gestational diabetes mellitus and obesity. Scientific Reports. 2021;11(1): 24373. doi: 10.1038/s41598-021-03779-7.
Xie W, Zhang H, Ni Y, Peng Y. Contrasting diversity and composition of human colostrum microbiota in a maternal cohort with different ethnic origins but shared physical geography (Island scale). Front Microbiol. 2022;13:934232. doi: 10.3389/fmicb.2022.934232.
Chande AT, Nagar SD, Rishishwar L, Mariño-Ramírez L, Medina-Rivas MA, Valderrama-Aguirre AE, Gallo JE. The impact of ethnicity and genetic ancestry on disease prevalence and risk in Colombia. Front Genet. 2021;12:690366. doi: 10.3389/fgene.2021.690366
Nascimento RC, Hochman VG, da Silva CB, do Valle BV, do Amaral Y, Dolinsky M, Vieira A. Immediate effect of food intake by the nursing mother on the macronutrient content of colostrum. J Pediatr. 2025. doi: 10.1016/j.jped.2025.03.004.
Londoño-Sierra DC, Mesa V, Guzmán NC, Bolívar Parra L, Montoya-Campuzano OI, Restrepo-Mesa SL. Maternal Diet May Modulate Breast Milk Microbiota—A Case Study in a Group of Colombian Women. Microorganisms. 2023;11(7):1812. doi: 10.3390/microorganisms11071812.
Damaceno QS, Souza JP, Nicoli JR, Paula RL, Assis GB, Figueiredo HC, Martins FS. Evaluation of potential probiotics isolated from human milk and colostrum. Probiotics Antimicrob Proteins. 2017;9: 371-379. doi: 10.1007/s12602-017-9270-1.
Tawi F. Advantages and limitations of potential methods for the analysis of bacteria in milk: a review. J Food Sci Technol 53, 42–49 (2016). doi: 10.1007/s13197-015-1993-y.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Luz A. Sarmiento-Rubiano, Leidys Goenaga, Marianella Suarez-Marenco, Clara Gutierrez-Castañeda, Carmen M. Sarmiento, Jimmy Becerra Enríquez
This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
