Identification by PCR-FRLP of bacteria present in the colostrum of women residing in Barranquilla, Colombia

Authors

DOI:

https://doi.org/10.17843/rpmesp.2025.422.14321

Keywords:

Colostrum, Polymorphism, Restriction Fragment Length, Microbiota, Breast Feeding

Abstract

With the aim of isolating and identifying bacteria present in the colostrum of women in the city of  Barranquilla, located in the Colombian Caribbean, we  carried out a descriptive study on 55 colostrum  samples, which were cultured on M17, MRS, and TOS agar and incubated under aerobic and anaerobic  conditions. A total of 350 microorganisms were isolated, of which 296 were identified at the genus level by PCR-RFLP with the enzymes HaeIII and RsaI. The online program kodebio.shinyapps.io/RFLP-inator was used for the in-silico identification of the isolates. Seven hypothetical bacterial genera were identified: Staphylococcus, Lactobacillus, Enterococcus, Bifidobacterium, Corynobacterium, Streptococcus, and Leuconostoc, with Staphylococcus, Lactobacillus, and Enterococcus being the most dominant, representing 61.1% of the identified microorganisms. Our results are a starting point for understanding the microbial composition of human colostrum, considering the particular context and environmental conditions of the Colombian Caribbean.

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Author Biographies

  • Luz Adriana Sarmiento-Rubiano, Grupo de Investigación Alimentación y Comportamiento Humano, Universidad Metropolitana, Barranquilla, Colombia

    Bacteriologa, MG, PhD. Investigador Universidad Metropolitana

  • Leidys Goenaga, Grupo de Investigación Gestión Ecológica y Agroindustrial, Programa de Microbiología, Universidad Libre, Barranquilla, Colombia

    Bacterióloga MG.

  • Marianella Suarez-Marenco, Grupo de Investigación Gestión Ecológica y Agroindustrial, Programa de Microbiología, Universidad Libre, Barranquilla, Colombia

    Microbióloga MG

  • Clara Gutierrez-Castañeda, Grupo de Investigación Gestión Ecológica y Agroindustrial, Programa de Microbiología, Universidad Libre, Barranquilla, Colombia

    Microbiologa MG.

  • Carmen M. Sarmiento, Medicina preventiva y comunitaria, Universidad del Sinú, Cartagena, Colombia

    Bacterióloga MG. PhD

  • Jimmy Becerra Enríquez, Grupo de Investigación Alimentación y Comportamiento Humano, Universidad Metropolitana, Barranquilla, Colombia

    Biólogo, MG, PhD

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Published

2025-06-18

Issue

Section

Brief Report

How to Cite

1.
Sarmiento-Rubiano LA, Goenaga L, Suarez-Marenco M, Gutierrez-Castañeda C, Sarmiento CM, Becerra Enríquez J. Identification by PCR-FRLP of bacteria present in the colostrum of women residing in Barranquilla, Colombia. Rev Peru Med Exp Salud Publica [Internet]. 2025 Jun. 18 [cited 2025 Jul. 16];42(2):184-9. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/14321