Infectivity of promastigotes in stationary phase of Leishmania (Viannia) braziliensis and Leishmania (Viannia) peruviana, in cell line DH82

Authors

  • Karen Daphne Calvay-Sánchez Facultad de Ciencias Biologicas. Universidad Nacional de Trujillo. Trujillo, Peru.
  • Nyshon Máximo Rojas-Palomino Laboratorio de Leishmaniosis, Centro Nacional de Salud Publica. Instituto Nacional de Salud. Lima, Peru.
  • Aidé Clorinda Sandoval-Juarez Laboratorio de Leishmaniosis, Centro Nacional de Salud Publica. Instituto Nacional de Salud. Lima, Peru.
  • Alberto Cisneros-Tarmeño Laboratorio de cultivo celular, Centro Nacional de Salud Publica, Instituto Nacional de Salud. Lima, Peru.
  • Cristian Obregón-Cahuaya Unidad de Analisis y Generacion de Evidencias en Salud Publica. Centro Nacional de Salud Publica. Instituto Nacional de Salud. Lima, Peru.
  • Gloria Sonia Minaya-Gómez Laboratorio de Leishmaniosis, Centro Nacional de Salud Publica. Instituto Nacional de Salud. Lima, Peru.

DOI:

https://doi.org/10.17843/rpmesp.2015.321.1572

Keywords:

Leishmaniasis, Virulence, Cell line, Leishmania braziliensis

Abstract

Objectives. To determine the infectivity of promastigotes of Leishmania (V.) peruviana and Leishmania (V.) braziliensis in monocyte-macrophage cell line DH82 of Canis familiaris. Materials and methods. Was conducted a experimental study during the months of january to december 2013. Were used strains of Leishmania were used (V.) braziliensis MHOM / PE / 84 / LC53 and Leishmania (V.) peruviana MHOM / PE / 84 / LC26. The cell line was infected with stationary phase promastigotes and infectivity was determined as the product of percent infected macrophages average amastigotes per macrophage observed in epifluorescence microscope. Results. 13% of metacyclic forms to Leishmania (V.) braziliensis corresponded to 17.5 days post inoculation and Leishmania (V.) peruviana a percentage of 9.5% on the day 14.5. No significant difference was found between infectivity of stationary phase promastigotes of both species. Conclusions. It is recommended assess the infectivity of metacyclic promastigotes peruviana strains of Leishmania (V.) and Leishmania (V.) braziliensis cell lines in order to determine the most appropriate model in vitro infection, allowing leishmanicidas make the drug more effective susceptibility studies for disease control.

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Published

2015-04-01

Issue

Section

Research Articles

How to Cite

1.
Calvay-Sánchez KD, Rojas-Palomino NM, Sandoval-Juarez AC, Cisneros-Tarmeño A, Obregón-Cahuaya C, Minaya-Gómez GS. Infectivity of promastigotes in stationary phase of Leishmania (Viannia) braziliensis and Leishmania (Viannia) peruviana, in cell line DH82. Rev Peru Med Exp Salud Publica [Internet]. 2015 Apr. 1 [cited 2024 Jun. 23];32(1):33-40. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/1572

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