Assessment of local and systemic inflammatory parameters of peripheral burn in an animal model

Authors

  • Wilmary Torres Unidad de Investigación de Lípidos y Lipoproteínas, Facultad de Ciencias de la Salud, Universidad de Carabobo. Maracay, Venezuela. Licenciado en Bioanálisis
  • Liseth Mendoza Unidad de Investigación de Lípidos y Lipoproteínas, Facultad de Ciencias de la Salud, Universidad de Carabobo. Maracay, Venezuela. Licenciado en Bioanálisis
  • Hember Vicci Unidad de Investigación de Lípidos y Lipoproteínas, Facultad de Ciencias de la Salud, Universidad de Carabobo. Maracay, Venezuela. Licenciado en Bioanálisis
  • Antonio Eblen-Zajjur Centro de Biofísica y Neurociencia, Facultad de Ciencias de la Salud, Universidad de Carabobo. Valencia, Venezuela. médico cirujano, doctor en Neurofisiología
  • María Navarro Unidad de Investigación de Lípidos y Lipoproteínas, Facultad de Ciencias de la Salud, Universidad de Carabobo. Maracay, Venezuela. Licenciado en Bioanálisis. doctor en Bioquímica.

DOI:

https://doi.org/10.17843/rpmesp.2016.334.2556

Keywords:

Burn, Edema, Leukocytes, Platelets, Fibrinogen

Abstract

To evaluate the edema volume and leukocyte, platelet, and fibrinogen count of peripheral burn in an animal model. The back left leg of Rattus norvegicus (experimental group) was placed in water at 60 °C for 60 seconds or at room temperature (control group). An analysis was carried out before and after the induced burn (at 4, 8, 12, and 24 h). The edema volume was determined by an orthogonal photo, the leukocyte and platelet counts were determined using automated equipment, and the fibrinogen count was determined using the gravimetric method. The maximum value of the edema was recorded at 4 h and leukocytes at 24 h. The platelet count did not vary at different post-edema time intervals. The fibrinogen level increased at 4 h and 24 h. In this animal model we induced systemic inflammation characterized by leukocytosis and elevated fibrinogen levels, combined with edema located at the induction area.

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Published

2016-12-13

Issue

Section

Brief Report

How to Cite

1.
Torres W, Mendoza L, Vicci H, Eblen-Zajjur A, Navarro M. Assessment of local and systemic inflammatory parameters of peripheral burn in an animal model. Rev Peru Med Exp Salud Publica [Internet]. 2016 Dec. 13 [cited 2024 Apr. 17];33(4):713-8. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2556

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