Diagnostic performance of the indirect immunofluorescence test for the detection of antibodies against HTLV-1
DOI:
https://doi.org/10.17843/rpmesp.2017.343.2635Keywords:
Human T-lymphotropic virus 1, Diagnosis, Fluorescent antibody technique, indirectAbstract
The objective of the study was to determine the diagnostic yield of the indirect immunofluorescence (IFI) test for the detection of antibodies against HTLV-1. A diagnostic test evaluation study was performed. HTLV-1-infected MT2 cells and HTLV-1-uninfected K-562 cells were cultured; then these cells were impregnated and fixed in sheets for immunofluorescence and faced to Peruvian sera. A total of 155 sera (80 HTLV-1-positive sera and 75 sera positive for other diseases) from the Peruvian Instituto Nacional de Salud were used. In addition, the parameters of repeatability (intra-laboratory) and reproducibility (in laboratories of the Peruvian coast, mountains and jungle) of the test were evaluated. The IFI test detected the presence of antibodies against HTLV-1 reaching a sensitivity of 98.75% (95% CI: 95.69 – 100.00%), a specificity of 98.67% (95% CI: 95.40 – 100.00%) and the Kappa index was 0.975. There were no false positives or false negatives; however, one undetermined result and one non-specific result were obtained. The test showed 100% qualitative agreement when performing the repeatability and reproducibility. The results obtained are comparable to the reference test. Therefore, the IFI test had a good diagnostic performance and would be useful for the confirmation of HTLV-1.Downloads
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Published
2017-09-29
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Brief Report
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Copyright (c) 2017 Revista Peruana de Medicina Experimental y Salud Pública
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1.
Romero-Ruiz S, Miranda-Ulloa E. E, Briceño-Espinoza R. Diagnostic performance of the indirect immunofluorescence test for the detection of antibodies against HTLV-1. Rev Peru Med Exp Salud Publica [Internet]. 2017 Sep. 29 [cited 2024 Oct. 15];34(3):459-65. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2635