Diagnostic performance of the indirect immunofluorescence test for the detection of antibodies against HTLV-1

Authors

  • Soledad Romero-Ruiz Laboratorio de Referencia Nacional Virus de Transmisión Sexual VIH/SIDA, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo microbiólogo doctora en Ciencias Biológicas
  • Eduardo Miranda-Ulloa E. Laboratorio de Referencia Nacional Virus de Transmisión Sexual VIH/SIDA, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. biólogo magíster en Microbiología
  • Ronal Briceño-Espinoza Laboratorio de Referencia Nacional Virus de Transmisión Sexual VIH/SIDA, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. bachiller en Tecnología Médica

DOI:

https://doi.org/10.17843/rpmesp.2017.343.2635

Keywords:

Human T-lymphotropic virus 1, Diagnosis, Fluorescent antibody technique, indirect

Abstract

The objective of the study was to determine the diagnostic yield of the indirect immunofluorescence (IFI) test for the detection of antibodies against HTLV-1. A diagnostic test evaluation study was performed. HTLV-1-infected MT2 cells and HTLV-1-uninfected K-562 cells were cultured; then these cells were impregnated and fixed in sheets for immunofluorescence and faced to Peruvian sera. A total of 155 sera (80 HTLV-1-positive sera and 75 sera positive for other diseases) from the Peruvian Instituto Nacional de Salud were used. In addition, the parameters of repeatability (intra-laboratory) and reproducibility (in laboratories of the Peruvian coast, mountains and jungle) of the test were evaluated. The IFI test detected the presence of antibodies against HTLV-1 reaching a sensitivity of 98.75% (95% CI: 95.69 – 100.00%), a specificity of 98.67% (95% CI: 95.40 – 100.00%) and the Kappa index was 0.975. There were no false positives or false negatives; however, one undetermined result and one non-specific result were obtained. The test showed 100% qualitative agreement when performing the repeatability and reproducibility. The results obtained are comparable to the reference test. Therefore, the IFI test had a good diagnostic performance and would be useful for the confirmation of HTLV-1.

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Author Biographies

  • Soledad Romero-Ruiz, Laboratorio de Referencia Nacional Virus de Transmisión Sexual VIH/SIDA, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo microbiólogo doctora en Ciencias Biológicas
    Responsable del Laboratorio de Referencia Nacional de VTS VIH/SIDA CNSP-INS.
  • Eduardo Miranda-Ulloa E., Laboratorio de Referencia Nacional Virus de Transmisión Sexual VIH/SIDA, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. biólogo magíster en Microbiología
    Analista - Investigador, responsable del área de serología del Lanoratorio de Referencia Nacional de VTS VIH/SIDA
  • Ronal Briceño-Espinoza, Laboratorio de Referencia Nacional Virus de Transmisión Sexual VIH/SIDA, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. bachiller en Tecnología Médica
    Analista del área de serología en el Laboratorio de Referencia Nacional de VTS VIH/SIDA.

Published

2017-09-29

Issue

Section

Brief Report

How to Cite

1.
Romero-Ruiz S, Miranda-Ulloa E. E, Briceño-Espinoza R. Diagnostic performance of the indirect immunofluorescence test for the detection of antibodies against HTLV-1. Rev Peru Med Exp Salud Publica [Internet]. 2017 Sep. 29 [cited 2024 Oct. 15];34(3):459-65. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2635

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