Caracterización molecular de la nucleoproteína del virus de la rabia en canes procedentes de Arequipa, Perú

Carina Rosario Mantari Torpoco; Alfredo Miguel Berrocal Huallpa; Abraham Omar Espinoza-Culupú; Ricardo Luis López-Ingunza

doi:10.17843/rpmesp.2019.361.3938

Authors

Carina Rosario Mantari Torpoco Laboratorio de Zoonosis Virales, Instituto Nacional de Salud. Lima, Perú. Médico veterinario
Alfredo Miguel Berrocal Huallpa Laboratorio de Sistemática y Diversidad Vegetal, Museo de Historia Natural, Universidad Nacional Mayor de San Marcos, Lima, Perú. biólogo, magíster en Mejoramiento genético de plantas.
Abraham Omar Espinoza-Culupú Laboratorio de Bacteriología, Instituto Butantan. São Paulo, Brasil. Programa de Posgrado Interunidades en Biotecnología. Universidade de São Paulo, Brasil. biólogo, magíster en Biología molecular
Ricardo Luis López-Ingunza Laboratorio de Zoonosis Virales, Instituto Nacional de Salud. Lima, Perú. médico veterinario, magíster en Medicina veterinaria preventiva

DOI:

https://doi.org/10.17843/rpmesp.2019.361.3938

Keywords:

RNA Virus, rabies virus, high-performance nucleotide sequencing, dogs, Peru

Abstract

Objective. To characterize the nucleoprotein (N) and establish the origin of the rabies virus in dogs coming from Arequipa. Materials and Methods. Thirty samples of nervous tissue from the departments of Arequipa and Puno were analyzed. Total RNA was extracted from the samples and cDNA was synthesized to amplify the nucleoprotein gene, sequence it, and perform bioinformatics analysis. Results. A defined group was formed with respect to the external group (European bat lyssavirus). This group was classified into two subgroups, one constituted by samples coming from Puno and Arequipa (subgroup A), and another one by samples from Puno (subgroup B), exhibiting a nucleotide identity of 99.9% in subgroup A. This group was classified in two subgroups, one constituted by samples coming from Puno and Arequipa (subgroup A), and another one by samples from Puno (subgroup B), observing a nucleotide identity of 99.9% in subgroup A. Conclusions. The groupings of viral sequences show that the cases of canine rabies reported in Arequipa are the result of the expansion of canine rabies from the endemic region of Puno.