Evaluación de métodos de concentración y purificación de Giardia spp. a partir de muestras coprológicas

Kathia Tarqui Terrones; Giovanna Ramirez Carranza; Maria Beltrán Fabián

doi:10.17843/rpmesp.2019.362.4151

Authors

Kathia Tarqui Terrones Laboratorio de Referencia Nacional de Enteroparásitos, Instituto Nacional de Salud. Lima, Perú Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú Bióloga, MSc en Epidemiología
Giovanna Ramirez Carranza Laboratorio de Referencia Nacional de Enteroparásitos, Instituto Nacional de Salud. Lima, Perú. Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. bachiller en Microbiología y Parasitología
Maria Beltrán Fabián Laboratorio de Referencia Nacional de Enteroparásitos, Instituto Nacional de Salud. Lima, Perú Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. bióloga

DOI:

https://doi.org/10.17843/rpmesp.2019.362.4151

Keywords:

Giardia spp., Sucrose, Density gradient, Tube sedimentation, Cell count

Abstract

The aim of this study was to compare different methods of concentration to recover the largest number of Giardia spp. cysts from coprological samples. One hundred (100) samples from national reference hospitals were analyzed and four parasitological methods were applied: spontaneous tube sedimentation concentration (TSET), Faust, single-phase sucrose gradient, and two-phase sucrose gradient. The two-phase sucrose gradient method was found to achieve significantly better results in cyst concentration (121,903 cysts/ml) and amount of debris (6%), compared to Faust methods (35,355 cysts/ml), spontaneous tube sedimentation concentration (20,145 cysts/ml), and single-phase sucrose gradient (18,702 cysts/ml). It is concluded that the most effective method for the concentration and purification of Giardia spp. cysts from coprological samples is the two-phase sucrose gradient method, which would facilitate in vitro culture of Giardia spp.