Mutant alleles associated to chloroquine and sulfadoxine-pyrimethanime resistance in Plasmodium falciparum of the Ecuador-Peru and Ecuador-Colombia borders

Authors

  • Nancy Arróspide Centro Nacional de Salud Pública. Instituto Nacional de Salud. Lima, Perú. Biólogo
  • Gisely Hijar-Guerra Centro Nacional de Salud Pública. Instituto Nacional de Salud. Lima, Perú. Biólogo
  • Doménica de Mora Sistema Nacional de Control de Enfermedades Transmitidas por Vectores Artrópodos. Quito, Ecuador. Biólogo.
  • César Eduardo Diaz-Cortéz Sistema Nacional de Control de Enfermedades Transmitidas por Vectores Artrópodos. Quito, Ecuador. Médico salubrista.
  • Raúl Veloz-Perez Sistema Nacional de Control de Enfermedades Transmitidas por Vectores Artrópodos. Quito, Ecuador. Médico epidemiólogo.
  • Sonia Gutierrez Centro Nacional de Salud Pública. Instituto Nacional de Salud. Lima, Perú. Biólogo.
  • César Cabezas _Sánchez Centro Nacional de Salud Pública. Instituto Nacional de Salud. Lima, Perú. Médico infectólogo.

DOI:

https://doi.org/10.17843/rpmesp.2014.312.47

Keywords:

Plasmodium falciparum, Drug resistance, bacterial, Chloroquine, Sulfadoxine, Pyrimethamine

Abstract

The frequency of mutations in pfCRT and DHFR/DHPS genes of Plasmodium falciparum associated with resistance to chloroquine and sulfadoxine-pyrimethamine was evaluated in 83 strains from the districts of Esmeralda and Machala, located on the borders of Ecuador-Peru and Ecuador-Colombia in 2002. Polymerase chain reaction (PCR), conventional and its variants, was used. Mutations in the pfCRT gene were found in more than 90% of the samples from Esmeralda and Machala. For the DHFR gene, 90% of the strains were mutant samples from Esmeralda, 3 were double mutations and 1 was a triple mutation. In Machala, 25% were simple mutant forms and 75% mixed mutant forms (wild forms/mutant). In conclusion, resistance to chloroquine has been fixed in strains carrying K76T pfCRT mutation, whereas genetic imprinting for resistance to pyrimethamine is evolving, particularly in the district of Esmeralda.

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Published

2014-07-02

Issue

Section

Research Articles

How to Cite

1.
Arróspide N, Hijar-Guerra G, de Mora D, Diaz-Cortéz CE, Veloz-Perez R, Gutierrez S, et al. Mutant alleles associated to chloroquine and sulfadoxine-pyrimethanime resistance in Plasmodium falciparum of the Ecuador-Peru and Ecuador-Colombia borders. Rev Peru Med Exp Salud Publica [Internet]. 2014 Jul. 2 [cited 2024 Jun. 23];31(2). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/47