Behavioural response of Aedes aegypti (Linnaeus, 1762) exposed to pyretrhoids insecticides of frequently use in public health

Abstract

Objectives. To assess the behavioural response of the F1 generation of Aedes aegyti (L) collected in the Metropolitan area of Monterrey (Nuevo Leon, Mexico) vs. three adulticides pyrethroids frequently used in public health. Materials and methods. We used a novel modular system called HITSS (High-Throughput Screening System) to evaluate two behavioral responses (contact irritation and spatial repellency), as well as the toxicity of three insecticides DDT, permeathrin and bifenthrin at different concentrations (0.025, 0.25, 25 and 250 nmol/cm2 ). Results. In the concentration 2.5 nmol/cm2 , DDT (4.3 ± 2.4) and permethrin (8.0 ± 1.4) were the insecticides that are more irritating effect (p <0.05); the bifenthrin requires 20 times higher doses to achieve similar effects. In spatial repellency, the three insecticides tested have had a similar responses at all concentrations; DDT: 7 to 14%, permethrin: 9 to 15% and bifenthrin: 19% to 27%. Permethrin and bifenthrin in 0.025 nmol/cm2 concentrations, effects knockdown above 34%, with a mortality rate 19%, DDT requires 10 times higher concentrations to achieve similar effects. Conclusions. HITTS system can be used to assess the behavioural response to insecticides.