Genetic diversity of Aedes aegypti in the central-upper Paraná Cross- Border axis in Paraguay

Authors

  • Sady C. Britez Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, San Lorenzo, Paraguay. Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Asunción, San Lorenzo, Paraguay. Bióloga, máster en Ciencias Biomédicas https://orcid.org/0000-0002-8573-5334
  • Leidi Herrera Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, San Lorenzo, Paraguay. Instituto de Zoología y Ecología Tropical, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela. bióloga, PhD en Ciencias https://orcid.org/0000-0001-8515-2078
  • María C. Ferreira Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, San Lorenzo, Paraguay. bióloga, máster en Desarrollo Local Sostenible https://orcid.org/0000-0003-1256-5028
  • Leticia M. Rolón Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, San Lorenzo, Paraguay. Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Asunción, San Lorenzo, Paraguay. biotecnóloga https://orcid.org/0000-0002-9962-5208
  • Vanessa Ruiz Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, San Lorenzo, Paraguay. Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Asunción, San Lorenzo, Paraguay. biotecnóloga https://orcid.org/0000-0003-3898-8870
  • Nilsa González-Brítez Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, San Lorenzo, Paraguay.Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Asunción, San Lorenzo, Paraguay.bióloga, PhD en Parasitología https://orcid.org/0000-0001-5562-9728

DOI:

https://doi.org/10.17843/rpmesp.2022.392.10709

Keywords:

Aedes, Genetic Polymorphism, Random Amplified Polymorphic DNA Technique, Mosquito Vectors, Surveillance, Paraguay

Abstract

Objective: To determine the genetic diversity of Aedes aegypti in the Central-Alto Paraná cross-border road corridor of Paraguay, an area that has reports of dengue cases. Materials and methods: Twenty adult females were selected from hatching Ae. aegypti eggs from households geolocated in the departments of Alto Paraná, Caaguazú, Cordillera and Central, between 2018 and 2019. DNA was extracted from the tissue of females for amplifying their polymorphic patterns by random amplification of polymorphic DNA by PCR (RAPD-PCR), using primers H3 and B03 in order to identify genetic parameters of population
diversity. The relationships between mosquito populations according to locality were observed by unpaired arithmetic mean analysis. We used DIVA-GIS 7.3.0 and MAXENT to analyze the suitable areas of actual and potential geographic distribution of these Ae. aegypti populations. Results: Forty loci were identified by RAPD-PCR profiling, with moderate gene differentiation (Gst = 0.12). The cross-border corridor presented bioclimatic conditions for the presence of variant populations of Ae. aegypti, with precipitation in the warmest quarter and mean temperature in the driest quarter being determinant in the distribution. Conclusions: There is evidence of moderate genetic diversity in Ae. aegypti populations from areas that have reported dengue cases in the cross-border road corridor linking the Central and Alto Paraná departments of Paraguay. The study of genetic variability of Ae. aegypti is very useful for entomo-epidemiological surveillance and evaluation of possible resistance to chemical control.

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Published

2022-06-30

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Section

Original Article

How to Cite

1.
Britez SC, Herrera L, Ferreira MC, Rolón LM, Ruiz V, González-Brítez N. Genetic diversity of Aedes aegypti in the central-upper Paraná Cross- Border axis in Paraguay. Rev Peru Med Exp Salud Publica [Internet]. 2022 Jun. 30 [cited 2024 Mar. 29];39(2):170-7. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/10709